摘要
为探索玉米秸秆饲用高值化,利用菌酶协同发酵对膨化玉米秸秆进一步深度生物前处理。以55%膨化微贮玉米秸秆为粗饲料,配制水分为45%的肉牛全混合日粮(TMR),分别进行未添加(ck组)、添加布氏乳杆菌(LB组)、复合纤维素酶(ME组)和菌酶(BE组)处理后,密封发酵30 d和有氧暴露7 d,取样分析营养成分、发酵品质、有氧稳定性和黄曲霉毒素B1(AFB1)含量。结果表明:添加菌、酶或菌酶发酵TMR组干物质含量显著低于ck组(P<0.05),ME组和BE组中性洗涤纤维、酸性洗涤纤维、纤维素均显著低于ck组和LB组(P<0.05),BE组半纤维素显著低于ck组(P<0.05);BE组pH最低,且LB、ME、BE组显著低于ck组(P<0.05);ck组与LB组水溶性碳水化合物含量显著低于ME组和BE组(P<0.05),BE组乳酸和乙酸含量显著高于其他组(P<0.05);ck组、LB组、ME组、BE组有氧稳定性分别为62,133,65,143 h,且LB组和BE组平均温度分别在有氧暴露3,4,5,6 d显著低于ck组和ME组(P<0.05);在有氧暴露3,5,7 d时,ck组黄曲霉毒素B1含量显著高于其他各组(P<0.05),且LB组和BE组显著低于ck组和ME组(P<0.05)。利用布氏乳杆菌和复合纤维素酶协同发酵膨化玉米秸秆型TMR,可有效降解TMR结构性碳水化合物,改善发酵品质,提高有氧稳定性,减少AFB1含量。
In order to explore high-value feeding of corn straw,further deep biological pretreatment of puffed corn stalk was carried out by the synergistic fermentation of bacteria and enzymes.In this experiment,55%expanded corn stalk was used as coarse feed,and beef cattle total mixed ration(TMR)with 45%water content was prepared.Beef cattle TMR with no addition(ck group),supplemented with Lactobacillus brucelli(LB Group),compound cellulase(ME group)and bacterialenzyme(BE group)were subjected to sealed fermentation for 30 days and aerobic exposure for 7 days,respectively.Samples were taken to analyze nutrients,fermentation quality,aerobic stability and aflatoxin B1(AFB1)content.The results showed that dry matter content of TMR group was significantly lower than that of ck group(P<0.05),neutral detergent cellulose,acid detergent fiber and crude fiber of ME and BE groups were significantly lower than those of ck and LB groups(P<0.05),and hemicellulose of BE group was significantly lower than that of ck group(P<0.05);pH value of BE group was the lowest,and that of LB,ME and BE groups was significantly higher than that of ck group(P<0.05);Content of water-soluble carbohydrate in ck and LB groups was significantly lower than that in ME and BE groups(P<0.05),and content of lactic acid and acetic acid in BE group was significantly higher than that in other groups(P<0.05);Aerobic stability of ck,LB,ME,BE groups was 62,133,65,and 143 h respectively,and mean temperature of LB and BE groups was significantly lower than that of ck and ME groups at 3,4,5 and 6 d of aerobic exposure respectively(P<0.05);At 3,5 and 7 d of aerobic exposure,content of AFB1 in ck group was the highest,which was significantly higher than that in other groups(P<0.05),and content of AFB1 in LB and BE groups was significantly lower than that in ck and ME groups(P<0.05).Using Lactobacillus brucelli and compound cellulase to ferment puffed corn stalk type TMR can effectively degrade structural carbohydrate of TMR,improve fermentation quality,improve aerobic stability and reduce content of AFB1.
作者
萨如拉
徐均钊
包呼格吉乐图
朱全胜
牛化欣
Sarula;XU Junzhao;BAO Hugejiletu;ZHU Quansheng;NIU Huaxin(College of Animal Science and Technology,Inner Mongolia Minzu University,Tongliao 028000,China;Agriculture and Animal Husbandry Technology Promotion Center of Inner Mongolia Autonomous Region,Hohhot 010000,China;Mengzhiyuan Breeding Professional Cooperative of Horqin Zuoyi Zhongqi,Tongliao 028100,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2023年第4期461-466,共6页
Journal of Jilin Agricultural University
基金
内蒙古自治区科技攻关项目(2020GG0108,2021GG0035)
内蒙古自然科学基金项目(2022MS03074)
内蒙古自治区高等学校青年科技英才支持计划项目(NJYT22054)
内蒙古民族大学肉牛科技创新团队项目
内蒙古自治区科技重大专项(2021ZD0009)。
关键词
发酵全混合日粮
菌酶协同
发酵品质
肉牛
膨化玉米秸秆
fermented total mixed ration
bacteria-enzyme synergism
fermentation quality
beef cattle
puffed corn stalk