摘要
Colorectal cancer(CRC)is one of the most common malignancies worldwide.Development of predictivemolecular markers may help to achieve the best outcome in clinic.The purpose of this study is to identify thedifferentially expressed genes and new potential predictive and prognostic molecular biomarkers for CRC.Inthis study,CRC and matched normal tissues acquired from the same patient were used to extract total RNA.The NanoString nCounter assay was applied to determine the differentially expressed genes.The results werethen validated by using the Cancer Genome Atlas data.Finally,we identified 27 genes that revealed significantcorrelation with CRC in the tumor tissue.Several genes in the pan-cancer panel showed significant differentialexpression,which were more universal than others in the CRC tissue.Since some of them have not been reportedas being directly related to CRC yet,future mechanism studies can be designed based on this study.Our studydemonstrated NanoString nCounter assay could serve as an alternative approach for gene expression analysis andidentified several unreported differently expressed genes in CRC patients,which may provide some importantclues for more in-depth study of CRC and serve as potential predictive molecular biomarkers for clinical diagnosisapplication.
作者
赵晖
文柏清
康亚妮
ZHAO Hui;WEN Baiqing;KANG Yani(Bio-ID Center,School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)
基金
the Natural Science Foundationof Shanghai(No.19ZR1476100)
the Interdisciplinary Program of Shanghai Jiao Tong University(Nos.YG2019GD02,YG2019QNB23,YG2019QNA49 and YG2019QNA52)。
