摘要
目的研究胃癌超声双重造影(DCEUS)参数与临床病理特征、miR-134-3p及潜在靶基因血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达的关系。方法选择2015年1月至2016年12月在河北中石油中心医院接受手术治疗的128例胃癌患者为研究对象,术前进行DCEUS,术中收集胃癌组织与癌旁组织,计算并比较各组胃癌组织及正常胃壁组织的造影剂达峰时间(TTP)、峰值强度(PI)、始增时间(RT);检测并比较不同DCEUS造影参数胃癌组织中miR-134-3p、VEGF、MMP-2、MMP-9的mRNA表达水平。术后随访无病生存期。结果与正常胃壁组织比较,胃癌组织的TTP、RT水平降低,PI水平增加(P均<0.05)。不同T分期、脉管侵犯情况的胃癌组织比较,TTP、RT、PI的差异均有统计学意义(P均<0.05)。与TTP及RT>中位数、PI≤中位数的胃癌组织比较,TTP及RT≤中位数、PI>中位数的胃癌组织中miR-134-3p的表达水平降低,VEGF、MMP-2、MMP-9 mRNA表达水平增加(P均<0.05)。与TTP及RT>中位数、PI≤中位数的胃癌患者比较,TTP及RT≤中位数、PI>中位数的胃癌患者无病生存期缩短(P<0.05)。结论胃癌DCEUS参数的变化与T分期增加、脉管侵犯的病理特征恶化及无病生存期缩短有关,也与miR-134-3p及潜在靶基因VEGF、MMP-2、MMP-9表达改变有关。
Objective To study the relationship among double contrast-enhanced ultrasonography(DCEUS)parameters of gastric cancer and clinicopathological features,the expression of miR-134-3p and potential target genes vascular endothelial growth factor(VEGF),matrix metalloproteinase-2(MMP-2)and MMP-9.Methods A total of 128 gastric cancer patients who received surgical treatment in Hebei Petro China Center Hospital from January 2015 to December 2016 were selected as the study objects.Preoperative DCEUS was performed,during the operation,gastric cancer tissue and adjacent tissue were collected,time to peak(TTP),peak intensity(PI),and response time(RT)of gastric cancer tissue and normal gastric wall tissue were calculated and compared.The mRNA expression levels of miR-134-3p,VEGF,MMP-2 and MMP-9 of gastric cancer tissue with different DCEUS parameters were detected and compared,and the disease-free survival was followed up after operation.Results Compared with normal gastric wall tissue,TTP and RT levels of gastric cancer tissue decreased,PI level increased(P all<0.05).TTP,RT,PI of gastric cancer tissues with different T stages and conditions of vascular invasion were significantly different(P all<0.05).Compared with gastric cancer tissue with TTP and RT>median,PI≤median,the expression level of miR-134-3p decreased,the mRNA expression levels of VEGF,MMP-2 and MMP-9 increased in gastric cancer with tissue TTP and RT≤median,PI>median(P all<0.05).Compared with gastric cancer patients with TTP and RT>median,PI≤median,the disease-free survival of patients with TTP and RT≤median,PI>median shortened(P<0.05).Conclusion The change of DCEUS parameters in gastric cancer relates to the deterioration of pathological features,including increased T stages and vascular invasion and the shortening of disease-free survival,also relates to the change of expression of miR-134-3p and potential target genes VEGF,MMP-2 and MMP-9.
作者
汤丽健
郑雪
李阳
张新成
董久兴
TANG Lijian;ZHENG Xue;LI Yang;ZHANG Xincheng;DONG Jiuxing(Department of Ultrasound Medicine,Hebei Petro China Center Hospital,Langfang 065000,China)
出处
《宁夏医科大学学报》
2023年第8期814-818,839,共6页
Journal of Ningxia Medical University
基金
河北省廊坊市科技支撑项目(2018013157)。
关键词
胃癌
超声双重造影
病理特征
血管内皮生长因子
基质金属蛋白酶
gastric cancer
double contrast-enhanced ultrasonography
pathological features
vascular endothelial growth factor
matrix metallo-proteinase