不同矿化水对纳米纤维素-铁螯合物的影响及其在矫治梨缺铁黄化中的应用

Effects of mineralized water on nanocellulose-iron chelates formulation and the application on plant iron deficiency chlorosis remediation

采用不同矿化度水质配制纳米纤维素(NC)-铁(Fe)螯合物(NC-Fe),研究不同矿化度水对NC-Fe的螯合及其对梨树缺铁黄化矫治的影响。结果表明:用矿化度为750、1500、3000 mg·L^(-1)的矿化水配制的NC-Fe悬浮液,其纳米粒子粒径、Zeta电位值随水矿化度的增加而变大;随水矿化度的升高,纳米螯合物的生物活性降低,与NC-Fe处理相比,由750、1500、3000 mg·L^(-1)矿化水配制的NC-Fe处理叶片活性铁含量分别下降9.1%、23.6、40.3%。此外,与NC-Fe处理相比,随水矿化度的升高,其配制的NC-Fe处理叶片内铁蛋白基因Fer1的相对表达量显著下降。综上,当水矿化度小于1500 mg·L^(-1)时,矿化度对NC-Fe的螯合有限,但仍能促进叶片有效铁含量提升,对铁蛋白基因Fer1的表达有诱导作用,故可在生产中使用Ⅲ类以下的矿化水配制NC-Fe,用于矫治梨树缺铁黄化。

This research studied the effects of different salinity levels of water on NC-Fe formulation and its application in remediation of iron deficiency chlorosis(IDC).The results showed that the particle size and Zeta potential of NC-Fe chelates were significantly increased with the increase of concentrations of mineralization level of water.Compared with NC-Fe formulated in 750 mg·L^(-1) of mineralization water(NC-Fe-750),the particle size and Zeta potential of NC-Fe chelated within 1500(NC-Fe-1500),3000 mg·L^(-1)(NC-Fe-3000)of mineralized water were increased.Compared with the treatment of NC-Fe formulated in DI water,the active iron in IDC leaves treated with NC-Fe-750,NC-Fe-1500 and NC-Fe-3000 decreased by 9.1%,23.6% and 40.3%,respectively.Moreover,compared with NC-Fe-DI,the relative expression of ferritin gene Fer1 in IDC leaves was decreased significantly with the increase of water mineralization in NC-Fe formulation.When the mineralization of water was less than 1500 mg·L^(-1),the salinity of water had limited chelation effect on NC-Fe,but it could promote the content of active iron in leaves and induce the expression of ferritin gene Fer1.Therefore,NC-Fe can be prepared with the mineralization of water below class Ⅲ to remediate IDC of pear trees.