基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为

Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning

用SiO_(2)纳米纤维作为三维结构刚性支撑框架,以聚乙烯醇(PVA)/丁烷四羧酸(BTCA)的酯化交联反应物为功能性有机黏合剂,制备了一种高度羧基化的海绵体整体吸附材料(PVA/BTCA多孔材料),得到的PVA/BTCA材料具有多孔结构.作为一种吸附材料,通过水下循环压缩性能测试证明PVA/BTCA材料在水下拥有良好的结构稳定性.PVA/BTCA多孔材料的高度羧酸化和高孔隙率为蛋白吸附提供了基础条件,可以选择性吸附带正电荷的蛋白.以溶菌酶为主要模型蛋白,PVA/BTCA多孔材料在pH=7时对溶菌酶的最大吸附量为1342.3 mg/g,PVA/BTCA多孔材料对溶菌酶的吸附的等温吸附模型与Freundlich吸附模型接近一致.优秀的机械稳定性和化学结构稳定性赋予PVA/BTCA多孔材料可重复使用性,这项工作有望为蛋白分离方向提供一种新的分离材料.

A highly carboxylated sponge monolithic adsorbent material(PVA/BTCA porous material)was prepared using SiO_(2) nanofibers as a three-dimensional structural rigid support framework and an esterified cross-linking reactant of poly(vinyl alcohol)(PVA)/butane tetracarboxylic acid(BTCA)as a functional organic binder.As a kind of adsorption material,it is proven that PVA/BTCA material has good structural stability under water by underwater cyclic compression test.The highly carboxylated and high porosity of the PVA/BTCA porous material provided the basis for protein adsorption,which could selectively adsorb positively charged proteins.Using lysozyme as the main model protein,the maximum adsorption of PVA/BTCA porous material for lysozyme at pH=7 was 1342.3 mg/g.The isothermal adsorption model of PVA/BTCA porous material for the adsorption of lysozyme is in close agreement with the Freundlich adsorption model.The excellent mechanical stability and chemical structural stability confer reusability to the PVA/BTCA porous material.This work is expected to provide a new separation material for protein separation.