基于高通量测序的蚊媒病毒检测

Detection of mosquito-borne viruses based on high-throughput sequencing

蚊虫在世界范围内分布广泛,体内携带高度多样性的病毒,其中蚊媒病毒可在吸血节肢动物及脊椎动物细胞中进行复制,能够感染人类或其他脊椎动物,具有严重的公共卫生危害。多年来,蚊媒病毒的监测主要依赖病毒分离培养等传统方法,而随着高通量测序技术的发展,不仅实现了对蚊虫病毒组本底的了解,还为蚊媒病原体的快速筛查、新病原体的发现以及蚊媒传染病的预防控制提供了基础。该文将详细介绍用于蚊媒病毒的高通量检测方法及其应用,以及高通量测序检测蚊媒病原体与传统方法相比的优势与劣势。

Mosquitoes are widely distributed around the world and carry a diverse array of viruses.Among these,mosquito-borne viruses possess the capability to replicate in both blood-sucking arthropods and vertebrate cells,rendering them significant threats to public health.For many years,the surveillance of mosquito-borne viruses mainly relied on traditional methods such as virus isolation and culture.The emergence of high-throughput sequencing(HTS)technology has not only revealed the entire spectrum of mosquito virome,but also provided a basic methodology for the rapid screening of mosquito-borne pathogens and identification of new species.Consequently,HTS has facilitated the prevention and control of mosquito-borne diseases.This review aims to describe the methods and application of HTS in the detection of mosquito-borne viruses,while also summarizing the advantages and disadvantages of HTS-based detection of mosquito-borne pathogens compared to traditional methods.