Trib3基因在大鼠睾丸组织不同发育时期的表达研究

STUDY ON THE EXPRESSION OF TRIB3 GENE IN RAT TESTICULAR TISSUE AT DIFFERENT DEVELOPMENTAL STAGES

目的研究Trib3基因在大鼠睾丸组织不同发育时期的表达。方法用RT-PCR、Western Blot、免疫组化等方法分别观察Trib3基因在大鼠精子生成过程中的表达情况。结果mRNA表达量在野生型大鼠出生后2~12 d较高,4 d达到高峰;14~16日龄与2~12日龄间差异无统计学意义(P>0.05);20~35日龄与14~16日龄比较,表达量显著降低,差异有统计学意义(P<0.05);40~65日龄与20~35日龄比较,表达量显著升高,差异有统计学意义(P<0.05)。Trib3蛋白在2~12日龄呈现较高表达;14~16日龄与2~12日龄比较,表达量显著降低,差异有统计学意义(P<0.05);20~35日龄与14~16日龄间Trib3表达量差异无统计学意义(P>0.05);40~65日龄与20~35日龄比较,表达量显著升高(P<0.05)。免疫组化结果可见Trib3主要表达于睾丸精原干细胞胞质部分及部分睾丸间质。结论Trib3基因可能参与了大鼠精原干细胞的增殖过程。

Objective To study the expression of Trib3 gene in rat testis at different periods.Methods The expression of Trib3 gene in rat spermatogenesis was observed by RT-PCR,Western Blot and immunohistochemistry.Results mRNA expres-sion in wild type rats was higher from 2 to 12 days after birth,and reached its peak on 4 days.There was no significant difference between 14~16 days of age and 2~12 days of age(P>0.05);The expression was significantly decreased at 20~35 days of age compared with 14-16 days of age(P<0.05);The expression level was significantly increased at 40~65 days of age compared with 20~35 days of age(P<0.05).Trib3 protein was highly expressed at 2~12 days of age.The expression level was significant-ly decreased at 14-16 days of age compared with 2~12 days of age(P<0.05);There was no significant difference in Trib3 ex-pression between 20~35 days of age and 14~16 days of age(P>0.05);The expression level was significantly increased at 40~65 days of age compared with 20~35 days of age(P<0.05).Immunohistochemical results showed that Trib3 was mainly ex-pressed in the cytoplasmic part of spermatogonial stem cells and some interstitium of testis.Conclusions Trib3 gene may be in-volved in the proliferation of spermatogonial stem cells.