药西瓜中3个新的葫芦烷型三萜苷及其抗炎活性研究

Three new cucurbitane-type triterpenoid glycosides from Citrullus colocynthis and their anti-inflammatory activity

综合运用多种色谱技术从药西瓜乙酸乙酯提取物中分离得到3个新的葫芦烷型三萜苷。利用核磁、高分辨质谱等技术及对照文献鉴定其结构为colocynthenin E(1)、colocynthenin G(2)、colocynthenin H(3)。通过LPS诱导的RAW264.7巨噬细胞炎症模型对新化合物1~3进行了抗炎活性的测定,结果表明,与阳性对照dexamethasone(IC_(50)=7.57μmol·L-1)相比,化合物2和3显示出微弱的抗炎活性,IC50分别为48.21、40.11μmol·L^(-1)。

Three new cucurbitane-type triterpenoid glycosides were separated from the ethyl acetate extract of Citrullus colocynthis by a variety of chromatographic techniques.According to the data of NMR,HR-ESI-MS,and/or comparison with the reported data,the three novel cucurbitane-type triterpenoid glycosides were identified as colocynthenin E(1),colocynthenin G(2),and colocynthenin H(3).The cell inflammation model was established with RAW264.7 macrophages exposed to lipopolysaccharide and then used to determine the anti-inflammatory activities of the three compounds.Compounds 2 and 3 showed mild anti-inflammatory activities with the IC_(50)of 48.21 and 40.11μmol·L^(-1),respectively,compared with that(IC_(50)=7.57μmol·L~(-1))of the positive control dexamethasone.