PRMT5及ASF1B在宫颈癌组织中的表达及临床意义

Expression and clinical significance of PRMT5 and ASF1B in cervical cancer

目的探讨宫颈癌组织中蛋白精氨酸甲基转移酶5(PRMT5)、组蛋白伴侣抗沉默功能蛋白1B(ASF1B)的表达及其与临床预后的关系。方法选择2018年1月至2019年1月该院收治的96例宫颈癌患者作为研究对象。采用免疫组织化学法检测癌组织和癌旁组织中PRMT5、ASF1B蛋白表达,Spearman秩相关分析癌组织中PRMT5与ASF1B蛋白表达的相关性。比较不同临床病理参数患者癌组织中PRMT5、ASF1B蛋白表达差异,Kaplan-Meier生存曲线分析PRMT5、ASF1B蛋白表达与预后的关系,单因素及多因素COX比例风险回归分析影响宫颈癌预后的因素。结果宫颈癌组织中PRMT5及ASF1B蛋白阳性率高于癌旁组织,差异均有统计学意义(P<0.05)。宫颈癌组织中PRMT5与ASF1B蛋白表达呈显著正相关(r=0.712,P<0.001)。国际妇产科联盟(FIGO)分期ⅠB2~ⅡA期、伴淋巴结转移患者癌组织中PRMT5、ASF1B蛋白阳性率分别高于FIGO分期ⅠA~ⅠB1期、无淋巴结转移患者,差异均有统计学意义(P<0.05)。PRMT5阳性及阴性表达组、ASF1B阳性及阴性表达组患者的3年总生存率比较,差异无统计学意义(P>0.05);PRMT5阳性表达组3年无进展生存率低于PRMT5阴性表达组,ASF1B阳性表达组3年无进展生存率低于ASF1B阴性表达组,差异均有统计学意义(P<0.001)。FIGO分期ⅠB2~ⅡA期、淋巴结转移及PRMT5、ASF1B阳性表达均是影响宫颈癌患者无进展生存率及预后的独立危险因素。结论宫颈癌组织中PRMT5、ASF1B表达升高,二者与FIGO分期、淋巴结转移有关,可作为宫颈癌预后相关肿瘤标志物。

Objective To investigate the protein expression of arginine methyltransferase 5(PRMT5)and histone chaperone anti silencing function 1B(ASF1B)in cervical cancer and their relationship with clinical prognosis.Methods Totally 96 patients with cervical cancer diagnosed and treated in the hospital from January 2018 to January 2019 were selected as the subjects.Immunohistochemistry was used to detect the expression of PRMT5 and ASF1B proteins in cancer tissues and adjacent tissues and Spearman rank correlation analysis was used to analyze the correlation between PRMT5 and ASF1B protein expression in cancer tissues.The expression of PRMT5 and ASF1B proteins in cancer tissues of patients with different clinical and pathological parameters was compared.In addition,Kaplan-Meier survival curve was used to analyze the relationship between PRMT5,ASF1B protein expression and prognosis.Univariate and multivariate COX proportional hazard regression analysis were used to analyze the factors influencing affecting the prognosis of cervical cancer.Results The positive rates of PRMT5 and ASF1B protein in cervical cancer tissues were higher than that in adjacent tissues,and the differences were statistically significant(P<0.05).There was a significant positive correlation between PRMT5 and ASF1B protein expression in cervical cancer tissues(r=0.712,P<0.001)The positive rates of PRMT5 and ASF1B protein in cancer tissues of patients with FIGO stageⅠB2-ⅡA and lymph node metastasis were significantly higher than those of patients with FIGO stageⅠA-ⅠB1 and without lymph node metastasis,respectively(P<0.05).There was no significant difference in 3-year overall survival rate between PRMT5 positive and negative expression groups,as well as ASF1B positive and negative expression groups(P>0.05).The 3-year progression-free survival rate of PRMT5 positive expression group was lower than that of PRMT5 negative expression group,and the 3-year progression-free survival rate of ASF1B positive expression group was lower than that of ASF1B negative expression group,with statistical significance(P<0.001).FIGO stageⅠB2-ⅡA,lymph node metastasis,PRMT5,ASF1B positive expression were all independent risk factors affecting 3-year progression-free survival rate and prognosis of cervical cancer patients.Conclusion The expression of PRMT5 and ASF1B increases in cervical cancer tissues,which are related to FIGO stage and lymph node metastasis,and could be used as tumor markers related to the prognosis of cervical cancer.