河鲀毒素诱导菊黄东方鲀膜转运蛋白基因的表达

Expression of membrane transporter gene of Takifugu flavidus induced by tetrodotoxin

为探究参与河鲀肝脏和皮肤累积河鲀毒素(Tetrodotoxin, TTX)的过程及相关调控基因,按每克体质量河鲀0.25μg TTX剂量尾部肌肉注射人工繁育的菊黄东方鲀(Takifugu flavidus),发现肝脏中TTX含量在注射后4 h时最高(19.12 ng/g),24 h下降到8.92 ng/g, TTX主要在肝实质细胞中累积;皮肤中TTX含量一直处于上升趋势,4 h为13.70 ng/g, 24 h为22.91 ng/g;TTX主要在皮肤表皮层、基底层细胞中累积。比较对照组和注射24 h后肝脏和皮肤转录组发现:TTX注射导致肝脏组织338个基因上调、350个基因下调;而皮肤组织有598个基因上调,同时有595个基因下调;RT-qPCR验证slc2a12、slc5a7、slc25a22、slc25a15、slc35a3、arap1、ugt2a2、ggt5、psbp2等膜转运蛋白相关基因在注射TTX后在肝脏和皮肤中的表达水平显著上调,可能与TTX累积过程有关。

In order to explore the process and related genes involved in the accumulation of TTX in the liver and skin of pufferfish,the artificially bred Takifugu flavidus(referred to as"pufferfish")underwent intramuscular injection of 0.25μg TTX per gram body weight of pufferfish.It was found that the concentration of TTX in the liver was the highest at 4 h after injection(19.12 ng/g),and decreased to 8.92 ng/g at 24 h,TTX was mainly accumulated in hepatocytes;TTX content in the skin was in an upward trend,and it was 13.70 ng/g at 4 h and 22.91 ng/g at 24 h;TTX mainly accumulated in the skin epidermis and basal layer cells.Comparing the control group and the liver and skin transcriptomes 24 h after injection,it was found that:338 genes were up-regulated and 350 genes were down-regulated in liver tissue by TTX injection,while 598 genes were up-regulated and 595 genes were down-regulated in skin tissue;RT-qPCR verified slc2a12,slc5a7,slc25a22,slc25a15,slc35a3,arap1,ugt2a2,ggt5,psbp2 and other membrane transporter-related genes were significantly up-regulated in the liver and skin after TTX injection(P<0.05),which may be related to the accumulation process of TTX.