硫乙醇酸盐优化培养基对嗜黏蛋白阿克曼菌生长及代谢产物的影响

Effect of Optimized Thioglycolate Medium on the Growth and Metabolites of Akkermansia muciniphila

本研究在硫乙醇酸盐液体培养基的基础上进行调整优化,嗜黏蛋白阿克曼菌(Akkermansia muciniphila,Akk)培养最终活菌数可达到10^(9)CFU/mL以上。同时,利用非靶向代谢组学技术对比分析两种培养基发酵上清液中的差异代谢物并对其中特征性产物进行了生理功能分析。结果表明,Akk利用优化后的培养基通过影响蛋白质的消化和吸收、氨酰基t RNA生物合成、胆汁分泌、ABC转运蛋白、氨基酸生物合成等代谢通路,产生更多的丙酸、巴多昔芬、多烯紫杉醇、长春花碱等多种功能性代谢产物,与抗癌、抗炎、减肥等功能直接相关。这一研究为Akk作为益生菌制剂及后生元对诸多疾病的调控功能性提供了确凿证据,同时为今后的工业化生产奠定了理论基础。

In this study,Akkermansia muciniphila(Akk)was cultured to a concentration of more than 10^(9) CFU/mL in optimized liquid thioglycolate medium.Besides,the differential metabolites in the fermentation supernatants from the original and optimized medium were analyzed by non-targeted metabolomics,and the physiological functions of the characteristic products were evaluated.The results showed that when cultured in the optimized medium,Akk produced higher amounts of various functional metabolites such as propionic acid,bardoxifene,docetaxel and vinblastine by affecting protein digestion and absorption,aminoacyl tRNA biosynthesis,bile secretion,ATP-binding cassette(ABC)transporter,and the metabolic pathways of amino acid biosynthesis.These metabolites could be directly related to anti-cancer,anti-inflammatory,anti-obese and other functions.This study provides solid evidence that Akk can function as a probiotic agent and postbiotic to regulate many diseases,and lays a theoretical foundation for future industrial production.