基于UPLC-MS非靶向代谢组学分析乳杆菌发酵方竹笋超细全浆的代谢差异

Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Mass Spectrometry for Analysis of Differential Metabolites in Lactobacillus-fermented Ultra-fine Whole Pulp of Chimonobambusa quadrangularis Shoot

利用超高效液相色谱-质谱技术对乳杆菌发酵方竹笋超细全浆进行非靶向代谢组学研究。以变量投影重要性(variable importance in projection,VIP)值大于1结合t检验P值小于0.05为标准进行筛选,结果发现,非发酵组(CK)和罗伊氏乳杆菌发酵组(LR)、CK组和植物乳杆菌发酵组(LP)、CK组和混菌发酵组(LRP)在正离子模式下分别筛选到361、392种和398种差异代谢物;在负离子模式下分别筛选到268、304种和321种差异代谢物。各组中差异代谢物种类主要有脂类及类脂类、有机酸及其衍生物、有机杂环化合物和苯类等;差异代谢物主要有组胺、D-甘露聚糖、吲哚-3-乳酸、DL-4-羟基苯乳酸、鸟氨酸和脯氨酸等。根据京都基因与基因组百科全书代谢通路富集分析得到4条显著的代谢通路,分别为嘌呤代谢、嘧啶代谢、苯丙氨酸代谢及柠檬酸循环。本研究探索了乳杆菌发酵方竹笋超细全浆的差异代谢物及代谢通路,为开发方竹笋相关发酵产品提供理论支撑。

In this work,a non-targeted metabolomic study was performed on the ultrafine whole pulp of Lactobacillusfermented Chimonobambusa quadrangularis shoot using ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS).Based on the variable importance in projection(VIP)>1 and P<0.05 in t-test,361,392 and 398 differential metabolites were selected in raw(control)versus Limosilactobacillus reuteri(LR)fermented samples,control versus Lactobacillus plantarum(LP)fermented samples,and control and LR+LP(LRP)fermented samples in the positive ion mode;and 268,304 and 321 differential metabolites in the negative ion mode,respectively.The major differential metabolites were lipids and lipid-like compounds,organic acids and their derivatives,organic heterocyclic compounds,and benzenes including histamine,D-mannan,indole-3-lactic acid,DL-4-hydroxyphenyl lactate,ornithine,and proline.Four significant metabolic pathways were identified by Kyoto Encyclopedia of Genes and Genomes metabolic pathway enrichment analysis,namely purine metabolism,pyrimidine metabolism and phenylalanine metabolism and citric acid cycle.This study provides theoretical support for the development of fermented bamboo shoot products.