高选择性比率型荧光探针用于急性肺损伤中次氯酸的检测

A Highly Selective Ratiometric Fluorescent Probe for the Detection of Hypochlorite in Acute Lung Injury

检测急性肺损伤中过表达的次氯酸要求荧光探针具有很高的选择性.合成了一种羟基萘香豆素探针NaphCou,可以高选择性比值型检测次氯酸,响应速度快,发射波长长(从绿色到红色变化),Stokes位移大(从107nm到155nm).利用该荧光探针,成功监测了脂多糖诱导急性肺损伤小鼠中次氯酸的浓度变化.

The fluorescent probe for the detection of overexpressed ClO-in acute lung injury is required to have high selectivity against other reactive species.A hydroxybenzo[g]coumarin,NaphCou,was synthesized as a ratiometric fluorescent probe to detect ClO-with ultra-high selectivity,a fast response,long-wavelength emissions(from green to red)and large Stokes shifts(from 107 nm to 155 nm).Using probe NaphCou as a fluorescence sensing tool,the lipopolysaccharide(LPS)-induced acute lung injury in mouse model was investigated by monitoring the hypochlorous acid concentration variation.