氟磺胺草醚胁迫红小豆幼苗代谢物及通路分析

Analysis of Metabolites and Pathways in Adzuki Bean Seedlings under Fomesafen Stress

为探究田间喷施氟磺胺草醚(FSA)对红小豆幼苗生长代谢的调控机制,以是否喷施FSA的红小豆幼苗为试验材料,采用液相色谱-质谱联用(LC-MS)代谢组学技术对红小豆幼苗代谢物的变化进行分析。结果表明,喷药的红小豆幼苗(Z-2-ZZ-2)组与未喷药红小豆幼苗(Z-2-ZZ)组相比,正离子模式下筛选出显著变化的差异代谢物106种(上调50种,下调56种),负离子模式下130种(上调42种,下调88种),正离子模式下注释到差异显著的代谢通路5条(嘧啶代谢、异黄酮生物合成、嘌呤代谢、半乳糖代谢、精氨酸和脯氨酸代谢),映射到差异代谢物13种,负离子模式下注释到差异显著的代谢通路2条(花青素生物合成和黄酮类生物合成),映射到差异代谢物5种。苯丙烷和聚酮化合物、脂质、类脂分子、有机酸及其衍生物类化合物在红小豆幼苗抵御FSA的胁迫中起主要作用。本研究为红小豆田间使用FSA的安全性评价及红小豆增收增产提供新思路。

To explore the regulatory mechanism of field spraying fomesafen(FSA)on the growth and metabolism of adzuki bean seedlings,using the adzuki bean seedlings sprayed with or without FSA as the experimental material,the metabolomics of adzuki bean seedlings was analyzed by liquid chromatography-mass spectrometry(LC-MS).The results showed that compared with the unsprayed adzuki bean seedlings(Z-2-ZZ-2)group,the significantly changed differential metabolites were screened,106 in the cation mode(50 up-regulated,56 down-regulated),130 in anion mode(42 up-regulated,88 down-regulated),five metabolic pathways with significant differences(pyrimidine metabolism,isoflavone biosynthesis,purine metabolism,galactose metabolism,and arginine and proline metabolism)were annotated in cation mode,mapped to 13 differential metabolites,and annotated to two significantly different metabolic pathways(anthocyanin biosynthesis and flavonoid biosynthesis)in anion mode,mapped to five differential metabolites.Phenylpropane and polyketides,lipids and lipid molecules,and organic acids and their derivatives played a major role in the resistance of adzuki bean seedlings to FSA stress.It provided new ideas for the safety evaluation of the use of FSA in the field of adzuki bean and for increasing the income and yield of adzuki bean.