基于SYRCLE工具与ARRIVE指南评价针刺干预高血压动物实验研究的质量

Evaluation of animal experiments on acupuncture for hypertension based on the SYRCLE’s tool and the ARRIVE guidelines

目的评价针刺干预高血压动物实验研究的偏倚风险与报告质量,分析实验设计、实施与报告中存在的不足,以减少动物实验偏倚风险和提高报告质量。方法计算机检索中国知网、万方、维普、中国生物医学文献数据库、Web of science、PubMed、Embase、The Cochrane Library等数据库,限制时间为建库至2022年10月8日,由两名研究员根据合格标准独立筛选针刺干预高血压动物的相关实验研究,并使用SYRCLE工具与ARRIVE指南2.0对纳入文献进行偏倚风险与报告质量评价。运用Excel 2019提取数据,根据结果进行描述性分析。结果共纳入79篇针刺干预高血压的动物实验文献,其中有17篇文献与21篇文献分别发表在中文核心/中国科学引文数据库收录期刊和SCI期刊上。SYRCLE评价工具显示,10个条目中有5个条目评估良好,其他条目均存在描述不充分或偏倚风险高的问题。在纳入的79篇文献中,19篇文献的偏倚风险较低,其余研究均存在一定的偏倚风险。ARRIVE指南2.0评价结果显示,38个亚条目中,有19个条目报告良好,而其他条目报告情况均较差。51篇文献的报告质量良好,其余研究报告不充分。结论目前,针刺干预高血压动物实验的偏倚风险较高,报告质量普遍偏低。由于多数重要条目描述不充分,导致实验可重复性和结果转化均受到影响。建议在实验设计和报告过程中参考SYRCLE工具与ARRIVE指南2.0,以提高针刺干预高血压动物实验的规范性与整体质量。

Objective To evaluate the risk of bias and reporting quality of animal experimental studies on the use of acupuncture for hypertension,and analyze the deficiencies in experimental design,implementation,and reporting,to reduce the risk of bias and improve the reporting quality of animal studies.Methods We searched the China National Knowledge Infrastructure,Wanfang,Chinese Science and Technology Periodical,Chinese Biomedical Literature,Web of Science,PubMed,Embase and The Cochrane Library databases from inception to October 8,2022.Two researchers independently screened experimental studies related to acupuncture interventions in hypertensive animals according to the eligibility criteria.The SYRCLE’s tool and the ARRIVE guidelines 2.0 were used to evaluate the risk of bias and reporting quality of the included studies.The data were extracted using Excel 2019 and a descriptive analysis was conducted.Results A total of 79 animal experiments on the use of acupuncture for hypertension were included,including 17 and 21 published in Chinese core/Chinese Science Citation Database-indexed journals and Science Citation Index journals,respectively.The result of SYRCLE’s tool analysis showed that five of the 10 items were evaluated well,while the rest had an unclear or high risk of bias.Of the 79 included articles,19 had a low risk of bias,while the remaining studies had some risk of bias.The result of the ARRIVE guidelines 2.0 indicated that 19 of the 38 sub-items were well reported,while the rest were poorly reported.The reporting quality was good for 51 articles but the remaining studies were inadequately reported.Conclusions The risk of bias in animal experiments of acupuncture intervention for hypertension is currently high and the reporting quality is generally low.The inadequate description of some important items affected the reproducibility of the experiments and the translation of the result.SYRCLE’s tool and ARRIVE guidelines 2.0 should be referred to during the experimental design and reporting of studies,thus improving the standardization and reporting quality of animal studies of acupuncture for hypertension.