尖锐湿疣患者皮损组织中Toll样受体4、核因子κB p65蛋白表达及临床意义

Expression and clinical significance of Toll-like receptor 4 and nuclear factor-κB p65 in skin lesions of patients with condyloma acuminatum

目的探讨尖锐湿疣患者皮损组织中Toll样受体4(TLR4)、核因子κB p65(NF-κB p65)蛋白表达及临床意义。方法选取2020年1月至2022年8月在江苏省南通市第三人民医院(以下简称“我院”)治疗的尖锐湿疣患者92例作为观察组,另选取同期于我院行包皮环切术的成年男性55例及外阴整形术的成年女性37例作为对照组,采用免疫组织化学染色检测观察组的皮损组织及对照组的皮肤组织中TLR4、NF-κB p65蛋白表达,比较两组TLR4、NF-κB p65蛋白表达的差异,并进一步分析观察组不同临床特征患者TLR4、NF-κB p65蛋白表达的差异。结果观察组TLR4、NF-κB p65蛋白表达阳性率高于对照组(P<0.05)。病程≥3个月、疣体数目≥10个、疣体最大直径≥10 mm患者的TLR4蛋白表达阳性率高于病程<3个月、疣体数目<10个、疣体最大直径<10 mm患者(P<0.05)。疣体数目≥10个、疣体最大直径≥10 mm患者NF-κB p65蛋白表达阳性率高于疣体数目<10个、疣体最大直径<10 mm患者(P<0.05)。结论尖锐湿疣患者皮损组织中TLR4、NF-B p65蛋白呈高表达,可能与疣体数目、疣体最大直径及病程有关。

Objective To investigate the expression and clinical significance of Toll like receptor 4(TLR4)and nuclear factor-κB p65(NF-κB p65)in skin lesions of patients with condyloma acuminatum.Methods Ninety-two patients with condyloma acuminatum treated in Nantong Third People’s Hospital of Jiangsu Province(hereinafter referred to as“our hospital”)from January 2020 to August 2022 were selected as the observation group,and 55 adult males who underwent circumcision and 37 adult females who underwent vulvoplasty in our hospital during the same period were selected as the control group.The expressions of TLR4 and NF-κB p65 protein in skin lesions of the observation group and skin tissues of the control group were detected by immunohistochemical staining.The differences in TLR4 and NF-κB p65 protein expression between the two groups were compared,and the differences in TLR4 and NF-κB p65 protein expression in patients with different clinical characteristics of the observation group were further analyzed.Results The positive rates of expression of TLR4 and NF-κB p65 in observation group were higher than those in control group(P<0.05).The positive rate of expression of TLR4 protein in patients with disease course≥3 months,number of warts≥10,and maximum diameter of warts≥10 mm were higher than those in patients with disease course<3 months,number of warts<10,and maximum diameter of warts<10 mm(P<0.05).The positive rate of expression of NF-κB p65 protein in patients with number of warts≥10 and maximum diameter of≥10 mm were higher than those in patients with number of warts<10 and maximum diameter of<10 mm(P<0.05).Conclusion The high expression of TLR4 and NF-κB p65 proteins in the skin lesions of patients with condyloma acuminatum may be related to the number of warts,the maximum diameter of warts,and the course of disease.