烟台市公共场所人工水环境中军团菌污染情况调查及其病原型别鉴定

Investigation of Legionella contamination in artificial water environment of public places and identification of disease prototypes in Yantai City

目的分析山东省烟台市公共场所人工水环境中军团菌污染情况及鉴别其病原型别。方法选取2019年1月至12月山东省烟台市的150份公共场所人工水环境标本为研究对象,对其军团菌检出率进行统计,对检出军团菌型别构成进行统计及比较,同时分析及比较不同水样、场所、菌落总数、季节及地点的军团菌检出情况。结果150份公共场所人工水环境标本中共有43份检出军团菌,检出率为28.67%。40份检出单一型别,3份检出混合型别,其中嗜血军团菌的检出率高于非嗜血军团菌,且以Lp1型占比高于其他型别,单一型别占比高于混合情况,差异有统计学意义(P<0.05);不同菌落总数样本的军团菌检出率比较,差异无统计学意义(P>0.05),而空调冷却水、医疗单位、夏季及郊区水样本的军团菌检出率高于其他水样、场所及季节的检出率,差异有统计学意义(P<0.05)。结论山东省烟台市公共场所人工水环境中军团菌污染情况较为突出,单一型别占比较高,且空调冷却水、医疗单位、夏季及郊区水样本的军团菌检出率相对更高,且不同水样、场所数及季节的检出情况存在较大差异,可作为干预与防控措施制订的参考依据。

Objective To analyze the Legionella contamination in artificial water environment of public places in Yantai City Shandong Province,and identification of its pathogenic type.Methods A total of 150 samples of artificial water environment in public places in Yantai City,Shandong Province from January to December 2019 were selected as the research objects,and the detection rate of Legionella was statistically analyzed,and the types of Legionella detected were statistically compared.Meanwhile,the detection situation of Legionella in different water samples,places,total number of colonies,seasons and locations were analyzed and compared.Results Among 150 samples from artificial water environment in public places,43 were detected with a detection rate of 28.67%,40 were detected with single type,and 3 were detected with mixed type.The detection rate of Legionella haemophilus was significantly higher than that of non-haemophilus Legionella,and the proportion of Lp1 type was higher than that of other types,and the proportion of single type was higher than that of mixed condition,and the differences were statistically significant(P<0.05).There was no statistical significance in the detection rate of Legionella in samples of different total colonies(P>0.05),while the detection rate of Legionella in samples of air conditioning cooling water,medical units,summer,and suburban water were higher than those of other water samples,places and seasons,and the differences were statistically significant(P<0.05).Conclusion In the artificial water environment of public places in Yantai City,Legionella contamination was prominent,with a high proportion of single types,and the detection rate of Legionella is relatively higher in air conditioning cooling water,medical units,summer,and suburban water samples,and there are great differences in the detection situation of different water samples,number of places and seasons,which can be used as a reference for formulating intervention and prevention and control measures.