基于HPLC-Q-TOF-MS技术分析益气生津袋泡茶的化学成分及入血成分

Analyses of chemical constituents and components absorbed into blood of Yiqi Shengjin Bag Tea by HPLC-Q-TOF-MS

目的采用HPLC-Q-TOF-MS技术对益气生津袋泡茶的主要化学成分及入血成分进行鉴定。方法6只8周龄SPF级雄性SD大鼠,体重280~300 g,采用随机数字表法分为给药组与空白组,每组3只。给药组灌胃4 ml益气生津袋泡茶水提物(生药浓度为1 g/ml),空白组灌胃4 ml生理盐水,2次/d,连续给药,第4天上午给药2 h后腹主动脉取血制备血清样品。采用Waters Xselect HSS T3 C18色谱柱(50 mm×2.1 mm,2.5μm),正、负离子模式下采集袋泡茶水提物与血清样品中的化学成分;以0.1%甲酸水-乙腈为流动相进行梯度洗脱。结果从益气生津袋泡茶水提物中鉴定出了17个化学成分,主要为人参皂苷类成分。同时,在血清样品中检测到了8个化学成分,分别为甜菜碱、人参皂苷Rg1、人参皂苷Re、哈巴俄苷、拟人参皂苷F11、人参皂苷Rb1、人参皂苷Rd、毛兰素。结论采用HPLC-Q-TOF-MS技术对益气生津袋泡茶中主要化学成分及入血成分进行定性分析有助于揭示益气生津袋泡茶的药效物质基础,为进一步的质量控制、药理学和活性机制研究提供参考。

Objective To identify the main chemical constituents and components absorbed into blood of Yiqi Shengjin Bag Tea by HPLC-Q-TOF-MS technology.Methods Six eight-week-old SPF male SD rats,weighing 280-300 g,were divided into administration group and control group by random number table method,with three rats in each group.In administration group,4 ml of Yiqi Shengjin Bag Tea water extract was intragastric(the crude drug concentration was 1 g/ml),while in blank group,4 ml of normal saline was intragastric,twice a day for continuous administration,and blood samples were collected from abdominal aorta 2 h after administration on the morning of the fourth day to prepare serum samples.A Waters Xselect HSS T3 C18 chromatographic column(50 mm×2.1 mm,2.5μm)was used to collect the chemical components of Yiqi Shengjin Bag Tea water extract and serum samples under positive and negative ion modes;gradient elution was performed with 0.1%formic acid-acetonitrile as mobile phase.Results Seventeen compounds were identified from Yiqi Shengjin Bag Tea water extract,mainly ginsenosides.Meanwhile,eight chemical constituents were identified from the serum sample,which were betaine,ginsenoside Rg1,ginsenoside Re,harpagoside,pseudoginsenoside F11,ginsenoside Rb1,ginsenoside Rd,and erianin.Conclusion HPLC-Q-TOF-MS technology was used to analyze the main chemical components of Yiqi Shengjin Bag Tea,which can help to reveal pharmacodynamic material basis of Shengjin Bag Tea,and provide reference for further quality control,pharmacology,and activity mechanism research.