黄脊竹蝗种群肠道微生物的多样性分析

Diversity analysis of gut microbes of yellow spined bamboo locust Ceracris kiangsu Tsai

昆虫肠道微生物是昆虫与自然环境互作的直接反应者。为探究不同地区黄脊竹蝗Ceracris kiangsu Tsai肠道微生物多样性和性别及环境因子的关系,本研究利用Illumina NovaSeq测序平台对新入侵地区和国内常发地区的黄脊竹蝗肠道微生物进行16S rDNA基因测序和分析。结果表明,地理位置和性别对黄脊竹蝗肠道菌群均存在影响,其中地理位置影响最为显著,其次为性别因素。通过Beta多样性分析发现采自湖南省邵阳市新宁县和云南省普洱市江城县的黄脊竹蝗肠道微生物差异性最大,3个地区不同性别样品的肠道菌群只有属水平组成和丰富具有显著差异。其中,湖南省益阳市桃江县的雌、雄样品优势菌属分别为乳球菌属Lactococcus(9.0%)和肠杆菌属Enterobacter(5.9%),云南省普洱市江城县的雌、雄样品优势菌属均为肠杆菌属(27.9%、27.0%),湖南省邵阳市新宁县的雌、雄样品优势菌属分别为肠杆菌属(29.9%)和乳球菌(12.0%)。所选取的8个环境因素中蒸发量、相对湿度和平均气压可能会影响黄脊竹蝗的肠道菌群多样性。研究结果从共生微生物和环境因子角度解析了黄脊竹蝗的环境适应性,为研制地理溯源技术和开发微生物制剂提供了科学依据。

Insect gut microbes are the direct responders of the interaction between insects and the natural environment.To explore the relationship between diversity of gut microbes and sex and environmental factors of C.kiangsu in different regions,the Illumina NovaSeq platform was used to sequence and analyze the 16S rDNA gene of C.kiangsu collected from newly invaded areas and frequently occupied areas in China.The results showed that geographical location and gender had effects on the diversity and composition of gut microbes,among which geographical location had the most significant effect,followed by developmental stage.The Beta-diversity analysis found that the gut microbes varied most in the diversity between Xinning County,Hunan Province,and Jiangcheng County,Yunnan Province.Samples with different genders showed significant differences in the composition and abundance of gut microbes only at the genus level in the three regions.Specifically,Lactococcus(9.0%)and Enterobacter(5.9%)was the most dominant genus in female and male samples of Taojiang County,Hunan Province;while the dominant counterparts in female and male samples of Jiangcheng county,Yunnan Province were both Enterobacter(27.9%,27.0%).Enterobacter(29.9%)and Lactococcus(12%)was the most dominant genus in female and male samples of Xinning County,Hunan Province.Among the eight selected environmental factors,the evaporation,relative humidity,and barometric pressure may influence the gut microbes'diversity.This study analyzed the environmental adaptability of C.kiangsu from the perspective of symbiotic microorganism and environmental factors,which will provide the basis for the development of geographical origin traceability technology and microbial-based locust management reagents.