基于DNA序列信息的贞琵甲属物种界定分析(鞘翅目:拟步甲科:琵甲亚科)

Molecular species delimitation of the genus Agnaptoria Reitter, 1887 based on DNA sequence (Coleoptera, Tenebrionidae, Blaptinae)

贞琵甲属Agnaptoria是小琵甲亚族Gnaptorinina中第三大属和青藏高原特有类群,已知36种/亚种。本文选取3个线粒体基因(COI;Cytb;16S rDNA)和1个核基因(28S rDNA-D2)片段,采用最大似然法(Maximum likelihood,ML)构建了该属的系统发育树;运用ASAP(Assemble Species by Automatic Partitioning)、GMYC(Generalized Mixed Yule Coalescent)和PTP(Poisson Tree Processes)3种方法对该属进行了分子物种界定分析。结果表明:综合运用3种分子物种界定方法的界定结果与形态鉴定结果基本吻合。依据形态特征与分子物种界定技术相结合的综合鉴定方法,大大提高了该类群的物种鉴定效率,为该类群未来在系统发育、地理分布格局演化等方面的研究提供了可靠的分子数据。

With 36 described species or subspecies,Agnaptoria Reitter is the third-most species-rich genus and endemic taxa of the Qinghai-Tibet Plateau in the darkling beetle subtribe Gnaptorinina(Tenebrionidae:Tenebrionina).In this study,phylogenetic analyses were reconstructed based on the concatenated dataset of one nuclear(28S rDNA-D2)and three mitochondrial genes(COI,Cytb,and 16S rDNA)under maximum likelihood(ML).We implemented multiple molecular species delimitation approaches(Assemble Species by Automatic Partitioning,ASAP;Generalized Mixed Yule Coalescent,GMYC;and Poisson Tree Processes,PTP)to reassess the status of Agnaptoria species.The results showed that three molecular species identification methods were consistent with those of morphological defined.The method of comprehensive identification of species based on morphological characteristics and molecular species delimitation technology has greatly improved the efficiency of species identification,this study provides valuable molecular data for phylogenetic and evolution of geographical distribution pattern studies.