cycle基因对噻嗪酮、噻虫嗪防治白背飞虱效果影响探索研究

Exploration of cycle affects buprofezin and thiamethoxam efficiency in Sogatella furcifera

白背飞虱Sogatella furcifera是我国重要的迁飞性水稻害虫,长期以来,其防治手段主要是化学防治。大量使用化学农药易导致环境污染及害虫抗药性增加等,因此需要探索高效的农药使用方法。本研究在1 d内不同时间点用0.27 g/L噻虫嗪、5 mL/L噻嗪酮对白背飞虱的致死率进行检测,结果发现,7∶00时噻虫嗪、噻嗪酮浸染72 h后的校正死亡率均最低,分别为54%、13.9%;16∶00时噻虫嗪、噻嗪酮浸染72 h后的校正死亡率均最高,分别为89.8%、44.9%。定量结果表明节律基因Sfcycle在白背飞虱雌虫或雄虫的中肠表达量最高,4龄和5龄若虫期在蜕皮前表达量比蜕皮后高,成虫期表达量相对稳定。RNAi研究结果发现Sfcycle能够影响白背飞虱P450基因SfCYP4DE1和SfCYP353D1v2的表达,用农药处理干涉后的白背飞虱死亡率都在7∶00、16∶00两个时间点都增高,且没有显著性差异,表明Sfcycle基因能够影响噻虫嗪、噻嗪酮对白背飞虱的防治效率。

Sogatella furcifera(white-backed planthopper,WBPH)is an important migratory rice pest in China,and chemical pesticides as the main method for a long time.The extensive use of chemical pesticides can lead to environmental pollution and increase insect resistance,so it is necessary to explore efficient pesticide application methods.In this study,0.27 g/L thiamethoxam and 5 mL/L buprofezin were used to detect the mortality rate of WBPH at different time points in a day.The results showed that adjusted mortality rates of thiamethoxam and buprofezin were the lowest at 7∶00,which were 54%and 13.9%respectively,and the corrected mortality rates after 72 h were the highest at 16∶00,89.8%and 44.9%respectively.The quantitative results showed that the expression level of the rhythm gene Sfcycle was the highest in the midgut of female or male of WBPH,and the expression level of the 4 th and 5th instar nymphs before molting was higher than that after molting,and the expression level was relatively stable in adult stage.RNAi results showed that Sfcycle could affect the expression of P450 genes SfCYP4DE1 and SfCYP353D1v2 of WBPH.The mortality of WBPH increased at 7∶00 and 16∶00 after the intervention of pesticide treatment,and there was no significant difference,which indicated that Sfcycle could affect the control efficiency of thiamethoxam and buprofezin.