柔性化、智能化、自动化细胞工厂

Flexible,intelligent,and automated artificial cell factory

“合成生命,设计生命”是合成生物学与生物制造的重要使命,通过按需设计、化学再造人工基因组,实现人工细胞工厂的精准构建。当前细胞工厂面临代谢网络复杂、缺乏理性设计、试错成本高、底盘生物改造周期长等瓶颈问题,限制了细胞工厂的应用。基于合成基因组学的柔性生物底盘,集成机器学习辅助智能设计、自动化高通量构建检验是潜在的解决途径,三者融合将构建下一代柔性化、智能化、自动化细胞工厂,打造分子生物合成的研发新范式,极大加速设计—合成—测试—优化的循环迭代,实现高价值目标分子的按需设计与快速生产。本文结合笔者近年在细胞工厂构建方面的研究工作,从国内外细胞工厂柔性化、智能化、自动化等方面进行综述,并展望未来发展趋势。

“Synthetic life,designed life”refers to the precise construction of artificial cells through on-demand design and chemical reconstruction of artificial genomes.At present,most cell factories are faced with bottleneck issues including complex metabolic network,shortage of rational design,high costs associated with trial and error,and long cycles for chassis bioengineering.On the other hand,flexible biological chassis technology is focused on integration of the following three aspects:assisted intelligent design,automated high-throughput construction,and testing technologies.In this way,cell factories can be constructed with flexible,intelligent,and automated characteristics.Such a breakthrough technology can not only greatly shorten the design-synthesis-testing-optimization cycle for the research of biological synthesis,but also facilitate the on-demand design and rapid production of high-value target molecules.Herein,we summarized the recent research work on cell factories regarding the flexibility,intelligence,and automation,and presented the prospects of cell factories in this hot research area.