肉苁蓉多糖对便秘型肠易激综合征大鼠的影响

Effects of Cistanches Herba polysaccharides on rats with constipation-predominant irritable bowel syndrome

目的探讨肉苁蓉多糖(简称CDPS)对便秘型肠易激综合征(IBS-C)大鼠的影响。方法将SD大鼠分为对照组(20只)和造模组。造模组大鼠灌胃0~4℃生理盐水2 mL(每天1次,连续14 d)诱导IBS-C模型。将造模成功的大鼠分为模型组、阳性对照组(枸橼酸莫沙必利,1.35 mg/kg)和CDPS低、高剂量组(50、100 mg/kg),每组20只。各药物组大鼠灌胃相应药液,对照组和模型组大鼠灌胃等体积水,每次1次,持续28 d。检测各组大鼠的粪便含水量、血清5-羟色胺(5-HT)含量、炭末推进率,观察其结肠组织病理形态并检测结肠组织中神经生长因子(NGF)、原肌球蛋白受体激酶A(TrkA)mRNA及蛋白的表达。结果与对照组比较,模型组大鼠的粪便含水量和炭末推进率均显著降低(P<0.05),结肠组织黏膜肌层断裂,炎症细胞浸润和细胞水肿明显,血清5-HT含量和结肠组织中NGF、TrkA mRNA及蛋白的相对表达量均显著升高(P<0.05)。与模型组比较,各药物组大鼠的粪便含水量和炭末推进率均显著升高(P<0.05),结肠组织病理改变明显减轻,血清5-HT含量和结肠组织中NGF、TrkA mRNA及蛋白的相对表达量均显著降低(P<0.05),其中,阳性对照组和CDPS高剂量组的上述指标普遍接近于对照组(P>0.05)。结论CDPS可缓解IBS-C大鼠的症状,该作用可能与NGF/TrkA信号通路被抑制有关。

OBJECTIVE To investigate the effects of Cistanches Herba polysaccharides(abbreviated as CDPS)on rats with constipation-predominant irritable bowel syndrome(IBS-C).METHODS SD rats were divided into control group(20 rats)and modeling group.The modeling group was given 2 mL of normal saline at 0-4℃intragastrically(once a day,for 14 consecutive days)to induce IBS-C model.After modeling,model rats were grouped into model group,positive control group(mosapride citrate,1.35 mg/kg),CDPS low-dose group(50 mg/kg)and CDPS high-dose group(100 mg/kg),with 20 rats in each group.Administration groups were given corresponding drug solution intragastrically,and control group and model group were given a constant volume of water intragastrically,once a day,for 28 consecutive days.The fecal water content,serum content of 5-hydroxytryptamine(5-HT),and charcoal powder propulsion rate of rats were determined in each group;the pathological morphology of colon tissue was observed,and mRNA and protein expressions of nerve growth factor(NGF)and tropomyosin receptor kinase A(TrkA)in colon tissue were detected.RESULTS Compared with control group,the fecal water content and carbon powder propulsion rate in the model group were decreased significantly(P<0.05);the rupture of mucosal muscle layer in colon tissue,significant infiltration of inflammatory cells and cellular edema were observed;the content of 5-HT in serum,and relative mRNA and protein expressions of NGF and TrkA were increased significantly(P<0.05).Compared with model group,the fecal water content and carbon powder propulsion rate of rats were increased significantly in administration groups(P<0.05),and pathological changes of colon tissue were relieved significantly,while the content of 5-HT in serum,the mRNA and protein expressions of NGF and TrkA in colon tissue were decreased significantly(P<0.05);among them,the above indicators in the positive control group and CDPS high-dose group were generally close to those in the control group(P>0.05).CONCLUSIONS CDPS can alleviate the symptoms of IBS-C rats,which may be related to the inhibition of NGF/TrkA signaling pathway.