基于UPLC法测定酱香型白酒原辅料中5种非挥发性有机酸

Determination of Five Non-Volatile Organic Acids in Raw and Auxiliary Materials of Jiangxiang Baijiu by UPLC

采用超高效液相色谱法(Ultra high performance liquid chromatography,UPLC)建立了酱香型白酒原辅料(高粱、大曲)中5种非挥发性有机酸(酒石酸、苹果酸、乳酸、马来酸、富马酸)含量的检测方法,结果表明外标法建立的5种非挥发性有机酸的标准曲线线性关系较好,其相关系数R~2不低于0.994,检出限在1.0~10.0μg/mL之间,加标回收率在92.4%~104.1%之间,精密度在0.1%~4.7%之间。该方法操作简单、稳定性好,可用于酱香型白酒原辅料中非挥发性有机酸含量的测定。

In this study a UPLC method was established for the determination of five non-volatile organic acids(tartaric acid,malic acid,lactic acid,maleic acid,fumaric acid) in the raw and auxiliary materials(sorghum,Daqu) of Jiangxiang Baijiu.The results showed that the standard curves of the five non-volatile organic acids established by the external standard method had good linear relationship,with the correlation coefficients R~2not less than 0.994.The limits of detection(LODs) were in the range of 1.0 μg/mL to10.0 μg/mL,the recoveries were in the range of 92.4 % to 104.1 %,and the precision was in the range of 0.1 % to 4.7 %.The method is simple and stable,and can be used for the determination of non-volatile organic acids in the raw and auxiliary materials of Jiangxiang Baijiu.