葡萄糖响应型丙烯基氧化石墨烯微凝胶Pickering乳液的制备及性能

Preparation and properties of glucose-responsive propylene-based graphene oxide microgels Pickering emulsion

以3-丙烯酰胺基苯硼酸(AAPBA)和丙烯基氧化石墨烯(GOM)为原料,采用自由基聚合法制备了GOM微凝胶,再以该微凝胶为乳化剂构建了Pickering乳液。对GOM微凝胶进行FTIR、SEM-EDS表征及葡萄糖响应性能测试。对GOM微凝胶Pickering乳液进行了荧光和粒径表征,并对其稳定性的影响因素进行了探究,以胰岛素为模型药物,对载药乳液的体外控制释药性能进行了评价。结果表明,成功合成了GOM微凝胶。GOM微凝胶中GOM添加量(以去离子水、AAPBA、丙烯酰胺、N,N’-亚甲基双丙烯酰胺、十二烷基硫酸钠、N,N-二甲基丙烯酰胺的总质量为基准,下同)为1.0%、GOM微凝胶添加量为水相质量的10.08%时,制备的水包油(O/W)型GOM微凝胶Pickering乳液稳定性最好,平均粒径为688.5 nm。GOM微凝胶稳定的载药Pickering乳液在不同浓度葡萄糖溶液中约5 h达到释药平衡,当葡萄糖的浓度增至40 mmol/L时,胰岛素的5 h累积释放率可达到94.21%。

Propylene-based graphene oxide(GOM)microgels were synthesized from free radical polymerization of 3-acrylamidophenylboronic acid(AAPBA)and GOM,and then emulsified to form Pickering emulsions.The GOM microgels obtained were characterized by FTIR and SEM-EDS,followed by analysis on its glucose-responsive performance.Investigations were conducted to analyze the fluorescent property and particle size distribution of the Pickering emulsion,the influencing factors on its stability as well as the in vitro controlled drug release performance of insulin-loaded emulsion.Results from characterization proved the successful synthesis of the GOM microgels.The oil in water(O/W)-type GOM microgel Pickering emulsion obtained showed the best stability with an average particle size of 688.5 nm with 1.0%of GOM(based on the total mass of deionized water,AAPBA,acrylamide,N,N'-methylene diacrylamide,sodium dodecyl sulfate,N,N-dimethylacrylamide,the same below)added in GOM microgels and 10.08%of GOM microgels mass in the aqueous phase.The GOM microgels-stabilized insulin-loaded Pickering emulsion reached drug release equilibrium around 5 h in glucose solutions with different concentrations.When the concentration of glucose was increased to 40 mmol/L,the cumulative release rate for 5 h of insulin could reach 94.21%.