SLC2A3和PD-L1在人胃腺癌组织中的表达及其临床意义

Expression and clinical significance of SLC2A3 and PD-L1 in human gastric adenocarcinoma

目的探讨人胃腺癌组织中葡萄糖转运蛋白2A3(SLC2A3)和程序性细胞死亡受体配体1(PD-L1)的表达情况及其临床意义。方法回顾性队列研究。收集2016年1月—2017年12月合肥市第八人民医院手术切除后病理检查确诊的50例胃腺癌标本及对应的癌旁组织。采用免疫组织化学方法检测胃腺癌组织标本中SLC2A3和PD-L1的表达情况,以及癌旁组织中SLC2A3的表达情况;分析胃腺癌组织中SLC2A3、PD-L1的表达与临床病理特征的关系,SLC2A3与PD-L1表达的相关性,以及SLC2A3、PD-L1表达情况与患者5年总生存情况的关系。结果胃腺癌组织中SLC2A3高表达率66.0%(33/50)高于癌旁正常组织8.0%(4/50),差异有统计学意义(χ^(2)=36.08,P<0.001)。胃腺癌组织中SLC2A3高表达与低表达在肿瘤分化程度、浸润深度、淋巴结转移及TNM分期方面差异均有统计学意义(P值均<0.05)。胃腺癌组织中PD-L1高表达率为62.0%(31/50),PD-L1高表达与低表达在淋巴结转移和肿瘤分期方面差异均有统计学意义(P值均<0.05)。相关性分析显示,SLC2A3表达情况与PD-L1表达情况呈正相关(rn=0.31,P=0.030)。50例患者术后随访6~60个月,随访期间患者死亡22例,术后5年总生存率56%(28/50)。5年总生存率比较:SLC2A3高表达患者为45.5%,低于SLC2A3低表达患者的76.5%;PD-L1高表达患者为73.7%,低于PD-L1低表达患者的45.2%;SLC2A3、PD-L1均高表达患者为41.7%,低于SLC2A3、PD-L1均低表达患者的90.0%:差异均有统计学意义(P值均<0.05)。结论胃腺癌中SLC2A3高表达率增高,SLC2A3与PD-L1的表达呈正相关,两者高表达与患者预后不良有关。

Objective This study aimed to investigate the relationship between solute carrier 2A3(SLC2A3)and programmed death receptor ligand 1(PD-L1)protein expressions in gastric adenocarcinoma and the clinical significance.Methods The retrospective cohort study was conducted.Fifty cases of gastric adenocarcinoma and surrounding normal mucosa tissues were collected from the Pathology Department of the Eighth People's Hospital of Hefei from January 2016 to December 2017.Immunohistochemical method was used to detect the expressions of the SLC2A3 and PD-L1 proteins in gastric adenocarcinoma and the surrounding normal mucosa tissues.This study analyzed the relationship between the expressions of the SLC2A3 and PD-L1 proteins and the clinicopathological significance in gastric adenocarcinoma tissues,the correlation between SLC2A3 and PD-L1 protein expressions,and the relationship between SLC2A3 and PD-L1 protein expressions and 5 year overall survival of patients.Results The high expression rate of the SLC2A3 protein in gastric adenocarcinoma tissues was 66.0%(33/50),which was higher than that in adjacent normal tissues(8.0%[4/50]),and the difference was statistically significant(χ^(2)=36.08,P<0.001).Significant differences were observed between the high and the low expressions of SLC2A3 protein in tumor differentiation degree,invasion depth,lymph node metastasis,and TNM stage(all P values<0.05).The high expression rate of the PD-L1 protein in gastric adenocarcinoma tissues was 62.0%(31/50).Significant differences were also observed between the high and the low expressions of the PD-L1 protein in the lymph node metastasis and tumor stage(all P values<0.05).Correlation analysis showed that the SLC2A3 protein expression was positively correlated with PD-L1 protein expression(rn=0.31,P=0.030).Fifty patients were followed up for 6-60 months,during which 22 patients died.The overall 5 year survival rate after surgery was 56%(28/50).The results of the comparison of the 5 year overall survival were as follows:45.5%of patients with high expression of SLC2A3 protein were lower than 76.5%of patients with low expression of SLC2A3 protein;73.7%of patients with high expression of PD-L1 protein were lower than 45.2%of patients with low expression of PD-L1 protein;41.7%of patients with high SLC2A3 and PD-L1 protein expressions were lower than 90.0%of patients with low SLC2A3 and PD-L1 protein expressions.The differences were statistically significant(all P values<0.05).Conclusion The high expression rate of the SLC2A3 protein is increased in gastric adenocarcinoma.The SLC2A3 and PD-L1 protein expressions are positively correlated,and their high expressions are associated with poor prognosis of patients.