拉曼光谱检测全细胞催化合成的3-氨甲基吡啶

Detection of whole-cell catalytic synthsised 3-(aminomethyl)pyridine by Raman spectroscopy

[目的]利用拉曼光谱,建立全细胞催化合成的3-氨甲基吡啶的检测方法。[方法]20 L发酵罐大肠杆菌发酵培养,5 L发酵罐全细胞催化,通过拉曼光谱检测产物3-氨甲基吡啶。[结果]拉曼光谱利用偏最小二乘法建立检测模型,所建模型校正集和预测集中预测值与实测值的线性决定系数分别为0.9412、0.9810,校正集和预测集均方根误差分别为0.53 mmol/L、0.41 mmol/L。[结论]经过配对样本t检验,液相检测值与拉曼预测值在显著性水平下没有显著性差异,利用拉曼光谱能够实现了3-氨甲基吡啶的实时、快速检测。

[Objective]To establish a method for the determination of 3-aminomethylpyridine concentration in whole-cell catalysis by using Raman spectroscopy.[Method]The product 3-aminomethylpyridine was detected by online Raman spectroscopy in 20 L fermenter of Escherichia coli and 5 L fermenter of whole cell catalysis.[Result]The linear coefficients of determination between the predicted value and the measured value in the calibration set and prediction set of the established model were 0.9412 and 0.9810,respectively.The root mean square errors of the calibration set and prediction set were 0.53 mmol/L and 0.41 mmol/L,respectively.[Conclusion]There was no significant difference between the liquid detection value and the predicted value at the significance level.The real-time and rapid detection of 3-aminomethylpyridine was realized.