基于氧化石墨烯-金纳米-葡萄糖氧化酶的新型葡萄糖比色传感器

A Novel Colorimetric Glucose Biosensor Based on Graphene Oxide-Gold Nanoparticles-Glucose Oxidase Composites

基于化学交联法和静电吸附法,制备了一种基于氧化石墨烯(GO)-金纳米颗粒(AuNPs)-葡萄糖氧化酶(GOD)的新型比色葡萄糖生物传感器。GOD会催化葡萄糖氧化生成H_(2)O_(2),生成的H_(2)O_(2)进一步诱导GO-AuNPs局部表面等离子体共振(LSPR)带的变化,从而实现葡萄糖的检测。GO改善了复合颗粒的稳定性,降低了金属颗粒的生物毒性,还改善了传感器的响应。通过透射电子显微镜(TEM),拉曼光谱和紫外可见分光光度计分别对复合颗粒的微观结构和光学性质进行了表征,并系统地研究了传感器的最佳检测条件,使开发的比色传感器对葡萄糖质量浓度具有高灵敏度和高选择性,并实测到传感器的灵敏度为14 nm/(mg/mL),范围为0~1.6 mg/mL,可用于实际检测。这项工作结合了GO和AuNPs的优势,显示了在葡萄糖检测领域简单,稳定和具有成本效益的应用前景。

A novel colorimetric glucose biosensor based on graphene oxide(GO)-gold nanoparticles(AuNPs)-glucose oxidase(GOD)is fabricated using chemical cross-linking and electrostatic methods.GOD catalyzes the glucose oxidation to produce H_(2)O_(2),which in turn shifts the local surface plasmon resonance(LSPR)band of GO-AuNPs to achieve glucose detection.GO improves the stability of composite particles,reduces metal particle biotoxicity,and enhances sensor response.The microstructure and optical properties of the composite particles are characterized using transmission electron microscope(TEM),Raman spectroscopy,and UV-Vis spectrophotometer.The optimal detection conditions of the sensor are systematically investigated,and the sensor exhibited high sensitivity and selectivity for tested glucose mass concentrations.The sensitivity of the sensor is 14 nm/(mg/mL)in the range of 0-1.6 mg/mL,which suggests that this sensor can be used for glucose detection in clinical settings.Our sensor combines the advantages of GO and AuNPs,and holds potential for simple,stable,and cost-effective use for glucose detection.