摘要
建立了小鼠原位肝癌模型,利用活体荧光成像系统激发的荧光信号对肿瘤进行了定位,通过光声显微成像系统观察了正常肝小叶、肿瘤中心和癌旁的微血管结构特征和血氧功能.结果表明,正常的血管分布均匀、分化良好,而肿瘤的血管分布不均匀且混乱,分支直径增加,更适合低氧环境.光声技术在研究肝细胞癌方面展现出巨大的潜力,可以为肿瘤抗血管生成治疗和诸多肝脏相关疾病的诊断提供更深入的见解.
Objective Hepatocellular carcinoma(HCC)is the most common primary liver malignancy and the second leading cause of cancer death worldwide.The development of HCC leads to abnormalities in the structure and function of blood vessels,which further lead to high pressure and hypoxia in the tumor microenvironment(TME).The most common clinical methods for identifying HCC nowadays are magnetic resonance imaging(MRI),computed tomography(CT),and ultrasound.MRI can measure multifunctional parameters of the liver;however,it has significant limitations in imaging resolution and is costly.CT can image the blood vessels in the liver;however,it uses X-ray,thus increases the risk of cancer.Ultrasound imaging is widely used to evaluate HCC;however,its sensitivity and specificity are low.Therefore,a more complete and reliable technique to analyze the micro-vascular morphology of HCC and TME is urgently needed.Photoacoustic imaging is a rapidly developing imaging technology in recent years.It offers a wide range of potential applications in the field of medical imaging and can visualize the structure and function information of biological tissues without labeling of contrast agents or invasion.Photoacoustic imaging has high specificity and sensitivity in the diagnosis of HCC and can visualize functional imaging of tumors and morphological examination of blood vessels.Methods A mouse model of in situ liver cancer was established,and the bioluminescence signal was activated by an in vivo fluorescence imaging system to locate the tumor.The microvascular structure characteristics and oxygen saturation of normal liver lobules,tumor centers,and adjacent tumors were accurately observed using photoacoustic microscopy.The concentrations of oxygenated and deoxygenated hemoglobins were quantified using the spectroscopic separation method to calculate blood oxygen saturation.The photoacoustic images were converted into binary images,and the vascular signals were extracted for density and diameter analysis.Results and Discussions The results obtained using photoacoustic microscopy via two wavelengths(532 nm/559 nm)show that the blood vessels in the normal liver are evenly spaced and well differentiated,whereas large irregular vessels appear at the edges of the tumors,and the vascular joints are curved and dilated.The blood vessels inside the tumors are unevenly distributed and the branch diameter increases.The oxygen concentration in the blood around the tumors decreases,resulting in a hypoxic and high-pressure TME.Conclusions In microvascular monitoring of hepatocellular carcinoma,the photoacoustic imaging can provide high-resolution images,which can more accurately detect the morphology of tiny and abnormal blood vessels,improving the accuracy of early cancer detection.Through image analysis,indicators such as the density and diameter of microvessels and other information such as oxygenation level and metabolic activity of tumor tissues can be evaluated to assess the growth state of tumors and predict the degree of malignancy.Photoacoustic imaging demonstrates a high application potential for studying the development of HCC.It can provide further insights into the antiangiogenic therapy of tumors and the diagnosis of numerous liver-related diseases.
作者
孙彤
黄国家
张振辉
Sun Tong;Huang Guojia;Zhang Zhenhui(MOE Key Laboratory of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou 510631,Guangdong,China;Guangdong Provincial Key Laboratory of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou 510631,Guangdong,China;Medical Research Institute,Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Southern Medical University,Guangzhou 510080,Guangdong,China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2023年第15期41-49,共9页
Chinese Journal of Lasers
关键词
医用光学
光声成像
肝细胞癌
肿瘤微环境
血氧饱和度
抗血管生成治疗
medical optics
photoacoustic imaging
hepatocellular carcinoma
tumor microenvironment
blood oxygen saturation
antiangiogenic therapy