蜂房药材中4种黄曲霉毒素的含量检测

Detection of Four Kinds of Aflatoxins in Nidus Vespae

建立高效液相色谱-免疫亲和柱净化-柱后光化学衍生法同时测定蜂房药材中黄曲霉毒素B_(1)、B_(2)、G_(1)、G_(2)的含量测定方法。样品采用70%甲醇超声处理30 min,经免疫亲和柱净化、高效液相色谱分离、光化学柱后衍生,通过荧光检测器测定4中黄曲霉毒素的含量。黄曲霉毒素B_(1)的线性范围为0.0104~0.0520 ng(r=0.9999)、黄曲霉毒素B_(2)的线性范围为0.0038~0.0190 ng(r=0.9998)、黄曲霉毒素G_(1)的线性范围为0.0108~0.0540 ng(r=0.9998)、黄曲霉毒素G_(2)的线性范围为0.0038~0.0190 ng(r=0.9998),线性关系良好。该方法检出限分别为0.42、0.15、0.43、0.15μg·kg^(-1),回收率在90.00%~99.49%之间,RSD≤3.1%(n=6)。该方法操作简便、灵敏度高、重复性好、结果准确,可用于蜂房中黄曲霉毒素含量的测定。

A determinationmethod of aflatoxin B_(1),B_(2),G_(1) and G_(2) in nidus vespae by immunoaffinity column clean-up and post-column photochemical derivatizationwasestablished.The sample was extracted with 70% methanol and purified by immunoaffinity columns.AflatoxinB_(1),B_(2),G_(1) and G_(2) in samples were anlyzed by HPLC-FLD with post-column photochemical derivatization.The linearity of aflatoxin B_(1) was at 0.0104~0.0520 ng(r=0.9999),the linearity of aflatoxin B_(2) was at 0.0038~0.0190 ng(r=0.9998),the linearity of aflatoxin G_(1) was at 0.0108~0.0540ng(r=0.9998),the linearity of aflatoxin G_(2) was at 0.0038~0.0190 ng(r=0.9998),the detection limits were0.42,0.15,0.43,0.15μg·kg^(-1),respectively.The recoveries were within 90.00%~99.49%with RSD≤3.1%(n=6).The method is easy to operate,has high sensitivity,good repeatability and accurate results.This method is suitable for the determination of AF in nidus vespae.