小菜蛾种群杂交对后代生长发育和解毒代谢酶活性的影响

Effects of Hybridization on Growth and Development and Detoxification Metabolic Enzyme Activity of Plutella xylostella Progenies

为明确敏感与抗性小菜蛾杂交对后代生长发育和抗药性的影响,探讨小菜蛾抗药性治理新思路。本研究选用室内敏感小菜蛾与田间抗性小菜蛾杂交,统计分析杂交后代F_(1)代小菜蛾生长发育、对药剂的敏感性及体内解毒代谢酶活性的差异变化。结果表明,小菜蛾敏感种群与抗性种群杂交对小菜蛾产卵量、发育历期、化蛹率和羽化率均无显著影响;小菜蛾正交和反交种群F_(1)代对茚虫威和乙基多杀菌素的敏感性均有提高,以正交小菜蛾F1代对茚虫威和乙基多杀菌素的抗性倍数降低更明显,与抗性自交种群相比,抗性倍数分别从27.47倍和41.00倍降低到6.82倍和21.50倍。小菜蛾杂交后代体内谷胱甘肽S-转移酶(GSTs)活性显著降低,正交和反交种群小菜蛾体内酶活性分别降低67.29%和53.80%。敏感与抗性小菜蛾杂交后,可能通过调控代谢酶活性影响小菜蛾对药剂的敏感性,研究结果可为小菜蛾的抗药性治理提供新思路。

The research aims to clarify the effect of hybridization between susceptible and resistant diamondback moth(DBM)on the growth,development and resistance of its progeny,and to explore new methods for the resistance management of DBM.In this study,the susceptible population hybridized with field resistant population of Plutella xylostella.The growth and development,sensitivity to pesticides and detoxification metabolic enzyme activity in F_(1) generation of diamondback moth were analyzed.The results showed that the hybridization of susceptible and resistant diamondback moth had no significant effect on the fecundity,developmental duration,pupation rate and emergence rate of Plutella xylostella.The sensitivity of DBM orthogonal and reverse cross populations F1 to indoxacarb and spinetoram was improved.The resistant ratio of DBM orthogonal F_(1) to indoxacarb and spinetoram decreased more obviously.Compared with the resistant population,the resistant ratio decreased from 27.47 or 41.00 times to 6.82 or 21.50 times,respectively.The activities of glutathione-s transferase(GSTs)in the hybrid offspring of diamondback moth decreased significantly.The enzyme activities of DBM orthogonal and reverse cross populations decreased by 67.29%and 53.80%,respectively.After hybridization between sensitive and resistant diamondback moth,the sensitivity of diamondback moth to pesticides may be affected by regulating the activity of metabolic enzymes.The results can provide a new idea for the management of diamondback moth resistance.