异养氨氧化细菌对多花黑麦草促生的影响

Effect of Heterotrophic AOB on the Growth-promoting of Lolium multiflorum

旨在揭示土壤氨氧化细菌(Ammonia oxdizing bacteria,AOB)菌株对多花黑麦草(Lolium multiflorum Lam.)的促生效果,以盆栽多花黑麦草为试验材料,共设置4个处理:未添加硝化抑制剂和未接种、接种AOB菌株后再生、添加硝化抑制剂后再生、添加硝化抑制剂后接种AOB菌株再生。结果表明,与未添加抑制剂和未接种再生相比,接种AOB菌株的多花黑麦草再生叶生物量增加23.53%~48.63%,叶片净光合率增加27.91%~48.54%,根际土壤硝化速率增加43.01%~144.21%;土壤中添加硝化抑制剂抑制了多花黑麦草的促生,而接种S2_8_1菌株后,多花黑麦草促生效果均优于添加硝化抑制剂处理。综上,接种S2_8_1后促进了多花黑麦草根际硝化作用,刺激NO_(3)^(-)含量的增加,光合作用增强,进而促进多花黑麦草的促生效果。

This study aimed to elucidate the growth-promoting effects of Ammonia oxidizing bacteria(AOB)strains on Italian ryegrass(Lolium multiflorum Lam.).Pot-grown Italian ryegrass was used as the experimental material,four treatments were established:no nitrification inhibitor and no inoculation,inoculation with AOB strains after regeneration,regeneration after adding a nitrification inhibitor,and inoculation with AOB strains after adding a nitrification inhibitor.The results showed that,compared to the non-inhibited and noninoculated regeneration,Italian ryegrass inoculated with AOB strains exhibited an increase of 23.53%to 48.63%in leaf biomass,27.91%to 48.54%in net photosynthetic rate,and 43.01%to 144.21%in rhizosphere soil nitrification rate.The addition of a nitrification inhibitor in the soil suppressed the growth promotion of Italian ryegrass.However,after inoculating with the S2_8_1 strain,the growth-promoting effects were superior to those of adding a nitrification inhibitor.In conclusion,the inoculation of S2_8_1 enhanced the nitrification process in Italian ryegrass rhizosphere,stimulated the increase of NO_(3)^(−)content,and strengthened photosynthesis,and ultimately promoted the growth-promoting effect of Italian ryegrass.