肝细胞癌患者血清中FOXA2与FOXJ2的表达及临床意义

Expression and clinical significance of FOXA2 and FOXJ2 in serum of HCC patients

目的:检测肝细胞癌(HCC)患者血清中叉头转录因子FOXA2与FOXJ2的表达水平,探讨FOXA2、FOXJ2与HCC患者肿瘤相关指标及无进展生存期的相关性。方法:选取河南中医药大学第一附属医院脾胃肝胆科2021年8月至2021年12月入院的HCC患者72人,收集患者血清。采用酶联免疫法检测HCC患者血清中FOXA2与FOXJ2的表达水平,分析其与HCC患者肿瘤相关指标及无进展生存期的相关性。计量数据与等级资料采用Spearman检验分析相关性;单因素相关性分析采用χ^(2)检验;运用ROC曲线计算FOXA2和FOXJ2曲线下面积(AUC),根据约登指数最大法得到截断值;采用Kaplan-Merier法进行无进展生存期分析。结果:FOXA2与FOXJ2表达水平均与肝癌临床分期相关(P<0.05),经ROC诊断曲线分析发现,FOXA2与FOXJ2的AUC对诊断存在统计学差异(P<0.05),经生存曲线分析发现FOXA2、FOXJ2高表达组HCC患者的累积生存率均高于FOXA2、FOXJ2低表达组HCC患者(P<0.05)。FOXA2与FOXJ2在血清中的表达水平存在中等程度的正相关关系(P<0.05)。结论:FOXA2、FOXJ2在HCC患者血清中的表达水平与HCC的发展及预后密切相关,可作为HCC判断疾病进展与预后的生物学标记物。

Objective:To detect the expression levels of forkhead transcription factors FOXA2 and FOXJ2 in sera of patients with hepatocellular carcinoma(HCC),and to explore the correlation between FOXA2 and FOXJ2 and tumor-related indicators and progression-free survival of HCC patients.Methods:Seventy-two patients with HCC who were admitted to the department of spleen,stomach,hepatobiliary,the First Affiliated Hospital of Henan University of Chinese Medicine from August 2021 to December 2021 were selected and their sera were collected.The expression levels of FOXA2 and FOXJ2 in the sera of HCC patients were detected by enzyme-linked immunoassay,and their correlation with tumor-related indicators and progression-free survival of HCC patients was analyzed.Correlations were analyzed by Spearman test for measurement data and rank data.χ^(2)test was used for one-way correlation analysis.ROC curve was applied to calculate the area under the curve(AUC)of FOXA2 and FOXJ2,and cut-off values were obtained according to the maximum method of Jorden index.Kaplan-Merier method was used for progression-free survival analysis.Results:Both FOXA2 and FOXJ2 expression levels were correlated with clinical stage of hepatocellular carcinoma(P<0.05),and the AUC of FOXA2 and FOXJ2 was found to be statistically different for diagnosis by ROC diagnostic curve analysis(P<0.05),and the cumulative survival rate of HCC patients in the FOXA2 and FOXJ2 high expression group was found to be higher than that of FOXA2 and FOXJ2 patients in the FOXJ2 low expression group by survival curve analysis(P<0.05).There was a moderately positive correlation between the expression levels of FOXA2 and FOXJ2 in the serum(P<0.05).Conclusion:The expression levels of FOXA2 and FOXJ2 in the serum of HCC patients are closely related to the development and prognosis of HCC,and can be used as biological markers for judging the disease progression and prognosis of HCC.