白细胞介素-6基因多态性与缺血性脑卒中的关联研究

Association between interleukin-6 gene polymorphism and ischemic stroke

目的探讨白细胞介素-6(interleukin-6,IL-6)基因多态性与缺血性脑卒中(ischemic stroke,IS)的关联性。方法选取2013年1月至2014年12月于新疆医科大学第一附属医院住院的367例维吾尔族急性IS患者纳入病例组,选取同期于本院参与维吾尔族心血管病流行病学调查的1202人纳入对照组,比较两组受试者的临床特征、IL-6基因rs1524107TC位点、rs2069840 CG位点、rs1800796 GC位点的基因型频率和等位基因频率;采用Logistic回归模型计算风险基因型对IS的相对风险度。结果两组受试者的性别、年龄、肥胖、高血压比例比较差异均有统计学意义(P<0.05)。两组受试者3个位点的基因型频率比较差异均有统计学意义(P<0.05);病例组患者的IL-6基因位点rs1524107 C等位基因频率高于对照组(P=0.005),两组受试者的rs2069840和rs1800796等位基因频率比较差异均无统计学意义(P>0.05)。rs1524107加性遗传模型能降低发病风险(OR=0.777,95%CI:0.641~0.941,P=0.010)。显性遗传模型下,IL-6基因rs1524107位点的TC基因型(OR=1.552,95%CI:1.210~1.989,P=0.001),rs1800796位点的GC基因型(OR=1.332,95%CI:1.051~1.689,P=0.018)能增加IS的发病风险;rs2069840位点的CG基因型(OR=0.739,95%CI:0.580~0.942,P=0.015)能降低IS的发病风险。结论IL-6基因rs1524107位点多态性可能是维吾尔族人群IS发病的保护因素,而rs2069840位点多态性可能是维吾尔族人群IS发病的危险因素。

Objective To investigate the association between interleukin-6(IL-6)gene polymorphism and ischemic stroke(IS).Methods A total of 367 Uyghur acute IS patients hospitalized in the First Affiliated Hospital of Xinjiang Medical University from January 2013 to December 2014 were included in case group.A total of 1202 Uyghur cardiovascular disease epidemiologists in our hospital during the same period were included in control group.The clinical characteristics,genotype frequency and allele frequency of rs1524107 TC site,rs2069840 CG site and rs1800796 GC site of IL-6 gene were compared between the two groups.Logistic regression model was used to calculate the relative risk degree of risk genotypes for IS.Results There were significant differences in gender,age,obesity and hypertension between the two groups(P<0.05).There were statistically significant differences in genotype frequency of 3 loci between the two groups(P<0.05).The allele frequency of IL-6 gene locus rs1524107 C in case group was higher than that in control group(P=0.005),and there was no significant difference in allele frequency of rs2069840 and rs1800796 between two groups(P>0.05).The additive genetic models of rs1524107 reduced the risk of IS(OR=0.777,95%CI:0.641-0.941,P=0.010).In the dominant genetic model,the genotype TC of rs1524107(OR=1.552,95%CI:1.210-1.989,P=0.001)and the genotype GC of rs1800796(OR=1.332,95%CI:1.051-1.689,P=0.018)could increase the risk of IS.Genotype CG of rs2069840(OR=0.739,95%CI:0.580-0.942,P=0.015)reduced the risk of IS.Conclusion rs1524107 polymorphisms of IL-6 gene may be protective factors for the incidence of IS in Uyghur population,while rs2069840 polymorphisms may be risk factors for the incidence of IS in Uyghur population.