艾曲泊帕治疗原发免疫性血小板减少症的药物经济学系统评价

Pharmacoeconomic systematic review of eltrombopag in treatment ofprimary immune thrombocytopenia

目的对艾曲泊帕治疗原发免疫性血小板减少症的药物经济学研究进行系统评价,为今后我国开展相关药物经济学评价提供参考。方法检索中国知网、万方、维普网、PubMed、Embase及Cochrane Library等数据库,根据纳入、排除标准,收集艾曲泊帕治疗原发免疫性血小板减少症的药物经济学研究。结果共纳入6篇文献,研究质量良好,对照措施均为罗米司亭。4篇研究显示,艾曲泊帕具有经济性;2篇研究显示,罗米司亭经济性优于艾曲泊帕。结论由于本研究纳入文献均来自国外,其药物经济学评价结果是否适用于我国还需进一步验证。亟需高质量的国内药物经济学研究,为我国临床合理用药提供依据。

Objective To investigate the effects of Eupatilin on the migration and invasion of human cervical cancer(CC)cells and its regulation on Notch signaling pathway.Methods The Caski cells,a human CC intestinal metastasis cell line,were cultured and divided into blank control(BC)group,negative control(NC)group,Eupatilin group,Notch 1 overexpression(Notch 1)group,Eupatilin and Notch 1 overexpression(Eupatilin+Notch 1)group.MTT method was performed to measure the viability of cells.Scratch healing test and Transwell test were performed to measure the migration and invasion of cells.Western blot was performed to measure the levels of epithelial-mesenchymal transition(EMT)and Notch signaling pathway-related proteins in cells.Results Compare with the NC group,the cell viability,migration and invasion abilities,N-cadherin,Snail,Notch 1,Hes-1 and Hes-5 protein levels in Eupatilin group were decreased,and the E-cadherin protein level was increased(P<0.05).The changes of the above indexes in Notch 1 group were opposite to those in Eupatilin group(P<0.05).The overexpression of Notch 1 reversed the effects of Eupatilin on the viability,migration and invasion of Caski cells and the Notch signaling pathway(P<0.05).Conclusion Eupatilin inhibits the migration and invasion of human CC cells through Notch 1/Hes signaling pathway,which may provide some new clues for the clinical treatment of human CC.