纳米复合材料免疫传感器的构建及其对肿瘤标志物CA72-4的检测

Construction of nanocomposite immunosensor and its detection of tumor marker CA72-4

为制备糖类抗原72-4(CA72-4)的高灵敏免疫传感器,以CoMOFs/CuAu为纳米材料,制备出一种基于CoMOFs/CuAu的电化学免疫传感器CA72-4/Ab1/CoMOFs/CuAu/GCE.利用扫描电镜和透射电镜对材料进行表征;同时采用循环伏安法(CV)、交流阻抗法(EIS)、差分脉冲伏安法(DPV)等检测方法对免疫传感器Ag/BSA/Ab/CoMOFs/CuAuGCE进行了电化学分析.结果表明:所构建的纳米材料免疫传感器在孵育时间为40 min、电解液pH为6.5条件下具有较宽的检测范围(0.001~100μmol/min·mL)和较低的检测限(0.007μmol/min·mL),其相关系数为R^(2)=0.99,实现了对肿瘤标志物的高灵敏检测;加标试验回收率在100.3%~106.6%之间,RSD值在2.36%~4.17%之间,该试验具有良好的选择性、重复性和稳定性.该研究为免疫传感器在临床应用提供了理论基础.

To prepare a highly sensitive immune sensor against carbohydrate antigen 72-4(CA72-4).In this study,using CoMOFs/CuAu as nanomaterials,an electrochemical immunosensor CA72-4/Ab1/CoMOFs/CuAu/GCE based on CoMOFs/CuAu was prepared.Scanning electron microscopy and transmission electron microscopy were carried out on the prepared nanomaterials.The detection methods such as cyclic voltammetry(CV),alternating impedance method(EIS),and differential pulse voltammetry(DPV)were used to convert biological signals into electrical signals,thereby electrochemical characterization and performance detection of immune sensors Ag/BSA/Ab/CoMOFs/CuAu/GCE were carried out.The results showed that the constructed nanomaterial immune sensor had a wide detection range(0.001~100μmol/min·mL)and a low detection limit(0.007μmol/min·mL)when the incubation time was 40 min and the electrolyte pH was 6.5,and its correlation coefficient was R^(2)=0.99,which realized the highly sensitive detection of tumor markers.The recovery was 100.3%106.6%and RSD was 2.36%~4.17%.The experiment had good selectivity,repeatability and stability.The recovery rate of spikes was between 100.3%and 106.6%,the RSD was 2.36%~4.17%,the experiment realizes highly sensitive detection of tumor markers,had good selectivity,repeatability and stability,which provides a theoretical basis for the clinical application of the sensor.