人体痛风石中微塑料的定性与定量分析

Qualitative and quantitative analysis of microplastics in human tophi

目的:检测微塑料是否存在于人体的痛风石中,并对检出的微塑料进行定性及定量分析,以进一步阐明痛风石的组成,探讨微塑料于关节处痛风石形成过程中可能起到的作用,为痛风治疗提供新思路。方法:收集5名男性痛风患者(血尿酸浓度>500μmol/L)的痛风石样品,采用热解-气相色谱/质谱法(Py-GC/MS)对痛风石中可能存在的10种微塑料成分进行检测分析。结果:4名患者的痛风石内检出大于或等于1种微塑料,共计4种不同类型的微塑料(聚苯乙烯、聚乙烯、聚氯乙烯、聚己二酰己二胺)被检出,且结果显示聚苯乙烯及聚乙烯二者检出率最高,平均含量分别为20.300和115.157 mg/kg。结论:微塑料可以在人体痛风石中被检出,其可能参与人体痛风石的形成。

OBJECTIVE:This study aimed at detecting whether microplastics existed in human tophi;and then qualitatively and quantitatively analyzing the detected microplastics;identifying compositions of tophi,exploring possible roles of microplastic accumulation in tophi formation in the joints,and then providing new ideas for the treatment of gout.METHODS:Samples of gouty tophus from 5 male patients(serum uric acid>500μmol/L)were included for analysis,and pyrolysis-gas chromatography-mass spectrometry(Py-GC/MS)was used to detect 10 types of microplastics that might exist in tophi.RESULTS:More than or equal to 1 type of microplastic was detected in the tophi of 4 patients,and a total of 4 different types of microplastics(polystyrene,polyethylene,polyvinyl chloride,polyadiohexylenediamine)were identified.The results demonstrate that polystyrene(PS)and polyethylene(PE)had the highest detection rate,with average contents of 20.300 and 115.157 mg/kg,respectively.CONCLUSION:Microplastics can be detected in human tophi,which may be involved in and may play a certain role in the formation of human tophi.