国内外青稞材料白粉病田间抗性鉴定

Resistance Identification and Evaluation to Powdery Mildew of Highland Barley Germplasm from Domestic and Overseas

本研究对国内外2 006份青稞种质材料进行了田间白粉病抗性鉴定,为青稞优异种质创制、抗病育种提供参考亲本。在田间诱导青稞发病,通过“0~9级法”对各青稞材料进行白粉病抗性等级评价;每一列供试材料间隔0.50 m作为诱发行,诱发行每隔0.50 m播种20粒“藏青13号”作为诱发材料。本研究对各青稞材料白粉病抗性进行了分类分析,抗性等级分为免疫(IM)、高抗(HR)、中抗(MR)、中感(MS)、高感(HS)和极感(VHS)6种,2 006份材料各抗病等级比例分别为25.47%,3.59%,6.28%,22.38%,41.77%和0.50%。本研究对大量青稞材料进行田间白粉病抗性鉴定,为深入研究青稞白粉病抗病机理和相关基因分子标记开发提供材料和数据支持。

In this study,the field powdery mildew resistance of 2,006 highland barley germplasm materials at home and abroad was identified,so as to provide reference parents for excellent germplasm creation and disease resistance breeding of highland barley.The disease was induced in the field,and the grade of powdery mildew resistance of barley materials was evaluated by"0~9 grade method".The interval of test materials in each row was 0.50 m,and 20 grains"Zangqing 13"as induced material were sown every 0.50 m in the induced row.The resistance of barley materials to powdery mildew was classified and analyzed.The resistance levels were divided into immune(IM),high resistance(HR),medium resistance(MR),medium sensitivity(MS),high sensitivity(HS)and extreme sensitivity(VHS).The proportions of disease resistance grades in 2,006 were 25.47%,3.59%,6.28%,22.38%,41.77%and 0.50%,respectively.In this study,a large number of barley materials were identified for field powdery mildew resistance,which provided material and data support for further research on the resistance mechanism of barley powdery mildew and development of related gene molecular markers.