椰子油对海南黑山羊羔羊瘤胃上皮发育的影响

Effects of Coconut Oil on Rumen Epithelium Development of Hainan Black Goat Lambs

为了探讨椰子油对羔羊瘤胃上皮发育的影响,该试验选用24只10日龄海南黑山羊羔羊,随机分成4个组,每组6个重复,在基础日粮中分别添加0(Ctrl组)、4 g/d(LCO组)、6 g/d(MCO组)和8 g/d(HCO组)椰子油,饲养周期90 d,集中屠宰采集瘤胃,分别测定瘤胃重量、体积,制备瘤胃上皮石蜡切片,光镜下检测瘤胃上皮发育情况。结果表明:MCO组羔羊瘤胃容积显著大于其它3组(P<0.05),瘤胃重和瘤胃指数无显著差异(P>0.05);LCO组和MCO组瘤胃上皮乳头表面积显著增加(P<0.05),HCO组上皮乳头密度与其它组相比极显著降低(P<0.01),椰子油对上皮乳头长度和厚度无显著影响(P>0.05);椰子油使羔羊瘤胃上皮乳头角质层(SB)细胞明显增多(P<0.05),LCO组和HCO组颗粒层(SG)细胞显著增多(P<0.05),MCO组和HCO组棘突层(SS)细胞显著增多(P<0.05)。结果提示,添加6 g/d的椰子油能使羔羊瘤胃的容积显著增加,一定程度刺激瘤胃上皮乳头细胞层的增生,促进羔羊瘤胃的发育。

The purpose of this study was to investigate the effects of coconut oil on rumen epithelial development of goat lambs.Twenty-four Hainan black goat lambs aged 10 days were used and randomly divided into 4 groups with 6 replicates in each group for a period of 90 days experiment.The basal diet was supplemented with 0(Ctrl group),4 g/d(group LCO),6 g/d(group MCO)and 8 g/d(group HCO)coconut oil,respectively.The rumen weight and volume were measured after slaughter,and the paraffin sections were prepared.The development of the rumen epithelium was detected under light microscope.The results showed that the rumen volume of lambs in MCO group was significantly higher than that in the other three groups(P<0.05).There were no significant differences in rumen weight and rumen index.The superficial area of ruminal epithelial papilla in LCO and MCO groups significantly increased(P<0.05),and the density of epithelial papilla in HCO group significantly decreased compared with other groups(P<0.001).The length and thickness of rumen papilla in HCO group had no significant effects(P>0.05).Coconut oil significantly increased stratum basale(SB),stratum granulosum(SG)in LCO and HCO groups(P<0.05),and stratum spinosum(SS)in MCO and HCO groups(P<0.05).The results suggest that 6 g/d of coconut oil could significantly increase the volume of rumen,stimulate the differentiation of epithelial papilla cell,and promote the development of rumen in lambs.