某猪场致生长育肥猪腹泻的猪圆环病毒2型(PCV-2)病原学检测与病理组织学观察

Pathogen Detection and Histopathological Observation of Porcine Circovirus Type 2(Pcv-2)Causing Diarrhea in Growing-Finishing Pigs in a Pig Farm

某猪场80日龄左右的生长育肥猪群出现顽固性腹泻,腹泻粪便的形状复杂,为了查明病原,该试验采集病猪病料,提取病毒DNA和RT-PCR检测,并进行病理变化分析和病理组织学观察。结果表明:实时荧光定量PCR检测显示猪圆环病毒2型(PCV-2)强阳性,剖检发现脾脏有轻微梗死、淋巴结萎缩,病理组织学观察发现回肠肠粘膜上皮细胞显著增生,由单层变为多层,淋巴结和脾脏的淋巴细胞数量显著减少。试验说明PCV2是引起该猪场育肥猪腹泻的主要病原。

The intractable diarrhea was found in fattening pigs about 80 days old on a pig farm,with complex shapes of feces.In order to identify the pathogen,the samples of diseased pigs were collected.Viral DNA was extracted,and virus detection was performed by RT-PCR method.Pathological changes and histopathological changes were observed and analyzed.The real-time fluorescence quantification results showed that PCV-2 was strongly positive.The autopsy found that there was a slight infarction in spleen and an atrophy in the lymph nodes.The histological observation revealed that the intestinal mucosal epithelial cells of the ileum were significantly proliferated,changing from a single layer to multiple layers.The number of lymphocytes in the lymph nodes and spleen was significantly reduced.This study showed that PCV-2 was the main pathogen causing diarrhea in fattening pigs.