外周血miR-200C、miR-101和CA-724联合检测对胃癌的诊断价值

Diagnostic value of combined detection of peripheral blood miR-200C,miR-101 and CA-724 on gastric cancer

目的:探讨外周血miR-200C、miR-101和糖类抗原-724(CA-724)联合检测对胃癌的诊断价值。方法:选取112例高度疑似胃癌患者为研究对象,根据病理检查结果分为胃癌组(n=69)和良性组(n=43);胃癌组患者再根据临床分期分为早期组(n=28)和晚期组(n=41),根据是否淋巴结转移分为转移组(n=43)和无转移组(n=26)。比较各组患者miR-200C、miR-101、CA-724水平,分析其对胃癌的诊断价值。结果:胃癌组患者miR-200C、miR-101低于良性组,CA-724高于良性组(P<0.05);早期组患者miR-200C、miR-101均高于晚期组(P<0.05),CA-724均低于晚期组(P<0.05);无转移组患者miR-200C、miR-101均高于转移组(P<0.05),CA-724低于转移组(P<0.05)。ROC曲线分析显示,miR-200C、miR-101、CA-724单独及三者联合诊断胃癌曲线下面积(AUC)分别为0.903、0.889、0.965、0.990,三者联合检测诊断的价值高于各指标单独检测(P<0.05)。结论:外周血miR-200C、miR-101在胃癌患者血液中表达呈下降趋势,CA-724则与之相反,三者联合检测对胃癌诊断价值较高。

Objective:To explore the diagnostic value of combined detection of peripheral blood miR-200C,miR-101 and carbohydrate antigen-724(CA-724)on gastric cancer.Methods:The clinical data of 112 patients with highly suspected gastric cancer were retrospectively analyzed,and the patients were divided into gastric cancer group(n=69)and benign group(n=43)according to pathological examination result.Patients in the gastric cancer group were further divided into early group(n=28)and advanced group(n=41)based on clinical staging,and divided into metastatic group(n=43)and non metastatic group(n=26)based on whether lymph node metastasis occurred.The levels of miR-200C,miR-101 and CA-724 were compared between groups,and the clinical value of the above indicators alone and in combination in the diagnosis of gastric cancer was evaluated.Results:miR-200C and miR-101 in gastric cancer group were lower than those in benign group,and CA-724 was higher than that in benign group(P<0.05).miR-200C and miR-101 in early group were higher than those in advanced group,and CA-724 was lower than that in advanced group(P<0.05).miR-200C and miR-101 in non metastatic group were higher than those in metastatic group,and CA-724 was lower than that in metastatic group(P<0.05).ROC curve showed that the area under curve(AUC)of miR-200C,miR-101,CA-724 alone and in combination in the diagnosis of gastric cancer were 0.903,0.889,0.965 and 0.990,respectively,and the combined diagnosis of the three was better than each indicator alone(P<0.05).Conclusion:Peripheral blood miR-200C and miR-101 show downward trends in the blood of patients with gastric cancer,while CA-724 shows the opposite trend.The combined detection of the three has higher diagnostic value for gastric cancer.