颈动脉粥样硬化斑块患者外周血LCAT基因启动子区DNA甲基化情况观察

DNA methylation in the promoter region of LCAT gene in peripheral blood of patients with carotid atherosclerotic plaque

目的观察颈动脉粥样硬化斑块患者外周血卵磷脂胆固醇酰基转移酶(LCAT)基因启动子区DNA甲基化情况。方法颈动脉粥样硬化斑块患者52例(观察组),同期无颈动脉粥样硬化斑块健康体检者50例(对照组),对所有受试者进行LCAT基因启动子区DNA甲基化检测,比较两组甲基化占比;对观察组患者进行头颈部CT血管成像(CTA)检查,比较观察组甲基化患者和非甲基化患者血管狭窄情况。结果观察组外周血LCAT基因启动子区DNA甲基化占比为65.38%(34/52),对照组外周血LCAT基因启动子区DNA甲基化占比为36.00%(18/50),两组甲基化占比比较,P<0.05。观察组34例甲基化患者中,颈动脉轻中度狭窄21例、重度及闭塞13例;观察组18例非甲基化患者中,颈动脉轻中度狭窄16例、重度及闭塞2例;观察组甲基化患者与非甲基化患者颈动脉狭窄严重程度比较,P均<0.05。结论颈动脉粥样硬化斑块患者外周血LCAT基因启动子区DNA甲基化占比高,LCAT基因启动子区DNA甲基化与动脉粥样硬化斑块的密切相关。

Objective To observe the DNA methylation in the promoter region of the lecithin cholesterol acyltransferase(LCAT)gene in peripheral blood of patients with carotid atherosclerotic plaque.Methods Fifty-two patients with carotid atherosclerotic plaque(observation group)and 50 healthy subjects without carotid atherosclerotic plaque(control group)during the same period were selected.DNA methylation in the promoter region of the LCAT gene was detected in all subjects to compare the difference of the methylation rate between the two groups.Head and neck CT angiography(CTA)examination was performed in the observation group.The degree of vascular stenosis was compared between methylated patients and non-methylated patients in the observation group.Results The DNA methylation rate in the promoter region of the LCAT gene in peripheral blood of the observation group was 65.38%(34/52),while that of the control group was 36.00%(18/50),with significant difference in the methylation rate between the two groups(P<0.05).Among the 34 methylated patients in the observation group,21 cases had mild-to-moderate carotid artery stenosis and 13 cases had severe stenosis or occlusion.Among the 18 non-methylated patients in the observation group,16 cases had mild-to-moderate carotid artery stenosis and 2 cases had severe stenosis or occlusion.There were significant differences in the severity of carotid artery stenosis between methylated and non-methylated patients in the observation group(P<0.05).Conclusion The DNA methylation rate in the promoter region of the LCAT gene in peripheral blood of patients with carotid atherosclerotic plaque is higher,and the DNA methylation in the promoter region of the LCAT gene is closely related to the formation of atherosclerotic plaque.