摘要
目的 :研究六味地黄丸对体外培养的黑素细胞的抑制作用。方法 :采用 MTT法测定细胞增殖情况 ;Na OH裂解法测定黑素合成 ;Takahashi法测定酪氨酸酶含量。结果 :六味地黄丸对黑素细胞的增殖有抑制作用 ,能使细胞数明显减少 (P<0 .0 5 ) ,并能使黑素合成显著下降 (P<0 .0 1) ,使酪氨酸酶活性逐渐减弱 (P<0 .0 1)。结论 :六味地黄丸能抑制黑素细胞增殖、黑素合成、酪氨酸酶活性 。
Objective:To study the mechanism and effect of liuwei dihuang pill on melanocytes Methods:MTT method,NaOH assay and Takahashi method were employed to measure the proliferation Results:liuwei dihuang pill induced a mild effect on melanocytic proliferation(P<0 05),while it showed a statistically significant decrease in the cell counts(P<0 01),melanin synthesis and tyrosinase activity(P<0 01) Conclusion:liuwei dihuang pill inhibits melanocytic proliferation,melanin synthesis(P<0 01),and tryosinase activity,which may be the mechanism that make it valid in clinical treatment of the pigmented skin diseases
出处
《山东中医杂志》
北大核心
2002年第12期733-735,共3页
Shandong Journal of Traditional Chinese Medicine
基金
湖北省自然科学基金资助项目 (2 0 0 0 J0 78)
