去整合素-金属蛋白酶17、Notch1及α-平滑肌动蛋白在子宫内膜异位症组织中的表达及意义

Expression and significance of ADAM17,Notch1 andα-SMA in endometriosis tissues

目的研究去整合素-金属蛋白酶(deintegrin metalloproteinase,ADAM)17、Notch1及α-平滑肌动蛋白(alpha-smooth muscle actin,α-SMA)在子宫内膜异位症(EMs)组织中的表达,及其与子宫内膜异位症的临床病理特征之间的相关性。方法选取2019年10月至2020年10月潍坊医学院附属医院收治的EMs病人异位内膜40例(异位内膜组)、在位内膜25例(在位内膜组),正常内膜25例作为对照组。采用免疫组化法及半定量分析法分别检测ADAM17、Notch1、α-SMA在三组中的表达。分析异位内膜组织中ADAM17、Notch1、α-SMA的表达与病理特征的关系。采用Pearson相关分析分析ADAM17、Notch1、α-SMA在三组中的表达的相关性。结果ADAM17、Notch1、α-SMA在异位内膜组中的表达高于在位内膜组(4.77±1.35比3.24±1.09,4.40±1.24比3.44±0.96,4.42±1.30比3.36±0.95),差异有统计学意义(P<0.05),在位内膜组的表达高于正常内膜组(2.36±1.22、2.24±1.01、2.16±1.028),差异有统计学意义(P<0.05)。异位内膜组中ADAM17、Notch1、α-SMA在Ⅲ~Ⅳ期中的表达高于Ⅰ~Ⅱ期,差异有统计学意义(P<0.05)。EMs病人异位内膜组中ADAM17、Notch1、α-SMA的表达与年龄及囊肿大小差异无统计学意义(P>0.05)。ADAM17、Notch1、α-SMA在异位内膜中的表达两两之间具有正相关性(P<0.05)。结论ADAM17、Notch1、α-SMA在EMs发生及发展中起重要作用,ADAM17可能通过Notch通路参与EMs的纤维化发生并调控EMs的纤维化严重程度。

Objective To investigate the expression of ADAM17,Notch1 andα-SMA in endometriosis tissues and their correlation with the clinicopathological features of endometriosis.Methods Forty cases of ectopic endometriosis(ectopic endometriosis group),and 25 cases of endometrium(endometrium group)in Affiliated Hospital of Weifang Medical University from October 2019 to October 2020 were collected,and 25 cases of normal endometrium in this hospital were selected as control group.The expressions of ADAM17,Notch1 andα-SMA in the three groups were detected by immunohistochemistry and semi-quantitative analysis.The relationship between the expression of ADAM17,Notch11,α-SMA and pathological features in ectopic endometrial tissue were analyzed.Pearson correlation analysis was used to analyze the correlation of ADAM17,Notch1 andα-SMA expression in the three groups.Results The expressions of ADAM17,Notch1andα-SMA in ectopic endometriosis group were higher than those in endometrium group(4.77±1.35 vs.3.24±1.09,4.40±1.24 vs.3.44±0.96,4.42±1.30 vs.3.36±0.95),and the differences were statistically significant(P<0.05).The expression of those proteins in endometrium group were higher than those in normal endometrium group(2.36±1.22,2.24±1.01,2.16±1.028),and the difference was statistically significant(P<0.05).The expressions of ADAM17,Notch1 andα-SMA in the ectopic endometriosis group in stageⅢ-Ⅳwere higher than those in stageⅠ-Ⅱ,and the differences were statistically significant(P<0.05).The expression of ADAM17,Notch1 andα-SMA in ectopic endometriosis group of EMs patients had no statistical significance with age and size of the cyst(P>0.05).The expression of ADAM17,Notch1 andα-SMA in ectopic endometriosis group was positively correlated(P<0.05).Conclusions ADAM17,Notch1 andα-SMA play an important role in the occurrence and development of EMs,and ADAM17 may participate in the fibrosis of EMs through Notch pathway.ADAM17 may regulate the severity of fibrosis in patients with EMs through Notch pathway.