桤木Genomic-SSR与EST-SSR分子标记的差异分析

Genetic Differences Revealed by Genomic-SSR and EST-SSR Markers in Alnus cremastogyne

桤木(Alnus cremastogyne)为非豆科固氮树种,也是我国最重要的一个桤木属特有种,具有重要的生态功能。本文分析了桤木基因组SSR与EST-SSR两种来源标记的遗传差异,结果显示EST-SSR的平均等位基因数与平均有效等位基因数高于Genomic-SSR,而对平均观察杂合度与平均期望杂合度来说,Genomic-SSR高于EST-SSR,聚类分析显示Genomic-SSR与EST-SSR在小的分群上有差异,说明Genomic-SSR与ESTSSR标记在解析遗传多样性与遗传关系方面有一定差异,综合两种标记可以获得更加客观的结果。

As a non-leguminous and nitrogen-fixing tree species,Alnus cremastogyne is also the most important endemic species of Alnus in China,which has important ecological functions.In this paper,the genetic differences of Genomic-SSR and EST-SSR markers in Alnus cremastogyne genome were analyzed.The results showed that the average number of alleles and the average number of effective alleles with EST-SSR were higher than those of genomic-SSR,while the average observed heterozygosity and average expected heterozygosity were higher than those of EST-SSR.Cluster analysis showed that there were differences between genomic-SSR and EST-SSR in small groups,which indicated that there were some differences between Genomic-SSR and EST-SSR in analyzing genetic diversity and genetic relationship to a certain extent,and more objective results could be obtained by combining the two marker methods.