基于网络药理学研究天南星活性成分及药理作用机制

Network Pharmacology-based Study on the Active Components and Pharmacological Mechanism of Arisaema Het⁃erophyllum Rhizoma

目的:基于网络药理学方法研究天南星的主要活性成分及药理作用机制。方法:通过TCMSP、UniProt、STRING数据库检索筛选天南星的活性成分、靶点、疾病,利用DAVID数据库进行京都基因与基因组百科全书(KEGG)和基因本体论(GO)富集分析,然后通过Cytoscape软件构建“成分-靶点-疾病”网络与蛋白质相互作用网络。结果:筛选出天南星6个活性成分,57个靶点,GO富集分析获得69个条目,KEGG获得47条信号通路。结论:通过网络药理学方法初步探究天南星的主要活性成分、作用靶点、生物过程和信号通路,并形成“中药-成分-靶点-通路”的完整作用过程,为进一步探讨天南星的药理作用机制提供了新的方向。

Objective:To study the active components and pharmacological mechanism of Arisaema heterophyllum Rhizoma(Tian Nan xing)based on network pharmacology approach.Methods:The active ingredients,targets,and diseases of Tennessee were screened by searching TCMSP,UniProt,and String databases,and the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)enrichment analyses were carried out by using the DAVID database,and then the"Ingredient-Target-Dis⁃ease"network and protein interaction network were constructed by Cytoscape software."Network and protein interaction network by Cytoscape software.Results:Six active components and 57 targets of Amanita muscaria were screened,69 entries were obtained by GO enrichment analysis,and 47 signaling pathways were obtained by KEGG.Conclusion:The main active ingredients,targets,biological processes and signaling pathways of Arisaema heterophyllum Rhizoma(Tian Nan xing)were initially investigated by the network pharmacology method,and the complete process of"TCM herbs-ingredient-target-pathway"was formed,which provides a new direction for further exploration of the pharmacological mechanism of Arisaema heterophyllum Rhizoma(Tian Nan xing).