妊娠糖尿病患者血清中FPR2的表达情况与Th1/Th2细胞平衡的相关性研究

Correlation between serum FPR2 expression and Th1/Th2 cell balance in patients with gestational diabetes mellitus

目的探讨妊娠糖尿病(GDM)患者血清中甲酰肽受体2(FPR2)的表达情况与辅助性T细胞1(Th1)/辅助性T细胞2(Th2)平衡的相关性。方法采用回顾性研究,将2020年1月至2022年1月于北京市顺义区妇幼保健院进行产检并住院分娩的52例GDM孕妇设为研究组,并选择同期在北京市顺义区妇幼保健院产检和分娩的健康孕妇60名作为对照组。采用实时荧光定量PCR检测两组孕妇血清中FPR2的相对表达水平;采用流式细胞术检测两组孕妇血清中Th1/Th2细胞比例;采用酶联免疫吸附试验检测两组孕妇血清中干扰素-γ(IFN-γ)、白细胞介素(IL)-2、IL-4、IL-10水平;采用Pearson法分析GDM患者血清中FPR2的表达水平与Th1/Th2细胞比例之间的相关性。结果研究组患者血清中FPR2、Th1表达水平分别为1.59±0.41、(9.59±2.56)%,均明显高于对照组[0.97±0.25、(8.61±2.02)%],Th2表达水平为(2.06±0.51)%,明显低于对照组[(3.28±0.88)%],且Th1/Th2细胞比例为5.11±1.02,高于对照组(3.20±0.79),差异均有统计学意义(P<0.05)。研究组患者血清中IFN-γ、IL-2水平分别为(27.96±5.21)、(16.69±4.02)pg/mL,均明显高于对照组[(20.19±4.59)、(14.12±3.86)pg/mL],而IL-4、IL-10水平分别为(11.46±2.36)、(31.52±6.24)pg/mL,均明显低于对照组[(13.09±3.01)、(38.81±7.16)pg/mL],差异均有统计学意义(P<0.05)。Pearson法分析结果显示,GDM患者血清中FPR2的表达与Th1/Th2细胞比例之间呈正相关(r=0.373,P<0.05)。结论GDM患者血清中FPR2高表达、Th1/Th2细胞比例升高,两者呈正相关,FPR2可能通过调节Th1/Th2细胞比例影响GDM的发生。

Objective To explore the correlation between the expression of serum formyl peptide receptor 2(FPR2)and the balance of T helper 1(Th1)/helper 2(Th2)cells in patients with gestational diabetes mellitus(GDM).Methods A retrospective study was conducted to establish a study group consisting of 52 GDM pregnant women who underwent prenatal examinations and were hospitalized for delivery at the Shunyi District Maternal and Child Health Hospital of Beijing from January 2020 to January 2022.And 60 healthy pregnant women who were in Shunyi District Maternal and Child Health Hospital of Beijing for obstetric examination and delivery were gathered as the control group.The relative expression level of FPR2 in the serum of pregnant women in two groups were detected by real-time fluorescence quantitative PCR.The ratio of Th1/Th2 cells in the serum of two groups of pregnant women was detected using flow cytometry.The levels of serum interferon-γ(IFN-γ),interleukin(IL)-2,IL-4,and IL-10 were detected by enzyme-linked immunosorbent assay.The correlation between the expression level of FPR2 in serum of GDM patients and the ratio of Th1/Th2 cells was analyzed by Pearson method.Results The expression levels of FPR2 and Th1 in the serum of patients in the study group were 1.59±0.41 and(9.59±2.56)%,respectively,which were significantly higher than those in the control group[0.97±0.25 and(8.61±2.02)%],the expression level of Th2 was(2.06±0.51)%,which was significantly lower than that in the control group[(3.28±0.88)%],the Th1/Th2 cell ratio was 5.11±1.02,which was higher than that in the control group(3.20±0.79),and the differences were statistically significant(P<0.05).The levels of IFN-γand IL-2 in the serum of patients in the study group were(27.96±5.21)and(16.69±4.02)pg/mL,respectively,which were significantly higher than those in the control group[(20.19±4.59)and(14.12±3.86)pg/mL],while the levels of IL-4 and IL-10 were(11.46±2.36)and(31.52±6.24)pg/mL,respectively,which were significantly lower than those in the control group[(13.09±3.01)and(38.81±7.16)pg/mL],and the differences were statistically significant(P<0.05).The results of Pearson analysis showed that there was a positive correlation between the expression of FPR2 in serum of GDM patients and the ratio of Th1/Th2 cells(r=0.373,P<0.05).Conclusion FPR2 is highly expressed in serum of GDM patients and the ratio of Th1/Th2 cells is increased,the two are positively correlated.FPR2 may affect the occurrence of GDM by regulating the ratio of Th1/Th2 cells.