拉曼光谱检测食源性致病菌生物被膜的研究进展

Advances in Raman spectroscopy detection for the biofilm offoodborne pathogens

生物被膜是菌群被其分泌的胞外聚合物所包裹的一种细菌生存定殖形式。食源性致病菌生物被膜具有不易检测和清除的特点,可能释放菌体进一步污染食品环境,导致消费者罹患食源性疾病风险上升,威胁食品安全。拉曼光谱是一种实时、快速、无损的检测技术,也被应用于食源性致病菌生物被膜的检测中。该文对应用拉曼光谱技术的生物被膜信息获取与分析工作进行了介绍,归纳了生物被膜胞外聚合物中多糖、脂质、蛋白质和核酸等生物大分子含量与代表性拉曼特征峰的对应关系,以及生物被膜在抗逆环境中基质、信号分子表现出的拉曼谱图变化,并讨论了拉曼光谱与其他检测技术联用检测生物被膜的可能。拉曼光谱技术在食源性致病菌生物被膜的精准检测方面虽仍具挑战,但可通过对检测过程的标准化、定量化,进一步扩大该技术在食品微生物安全领域的应用范围。

Biofilms is a form of bacterial survival and persistence that flora enfolded by its produced extracellular polymers substances(EPS).The biofilm of foodborne pathogens is difficult to detect and eliminate,which may raise the risk of food-borne diseases and compromise food safety by releasing pathogen cells into foods and environments.Raman spectroscopy is a real-time,non-destructive detection technology,which has been used in the detection of biofilm.This paper introduces the progress of obtaining and analyzing biofilm information using Raman spectroscopy.Then we summarized the relationship between the representative Raman characteristic peaks and the contents of carbohydrates,lipids,proteins,nucleic acids,and other biological macromolecules in EPS,as well as their changes under stress.The future detection on biofilm is also discussed by considering the combination of Raman spectra with other detection techniques.Although accurate detection for the biofilm of foodborne pathogens by Raman spectroscopy remains challenges,its application in food microbiological safety can be further expanded by standardizing and quantifying the detection procedure.