摘要
A turbot(Scophthalmus maximus)cell line named SMSP was obtained from the spleen.The origin of the cells was identified by morphology,chromosome number and COI gene.The optimal basic medium,serum concentration and growth temperature of the cells were detected.SMSP cell line is mainly composed of fibroblast-like cells.Most of the SMSP cells contained 44 chromosomes,and the sequence of COI gene confirmed that the cells were originated from turbot.The optimal culture conditions were 24℃,DMEM+10%FBS.The cell line had high transfection efficiency for siRNA and plasmid.After stimulation with lipopolysaccharide(LPS)or poly(I:C),the expressions of immune-related genes such as TNF-β,IL-12s,IL-10 and IL-1βwere up-regulated significantly in the early stage(P<0.05).This study will provide a model for exploring immune mechanism of turbot against pathogen in vitro.
基金
the National Natural Science Foundation of China(No.31902403)
the Young Experts of Taishan Scholars(No.tsqn201909130)
the advanced Talents Foundation of QAU grant(No.663-1120029)
the Shandong Technical System of Fish Industry(No.SDAIT-12-03)
the Breeding Plan of Shandong Provincial Qingchuang Research Team(2019),China。
