复方沙棘籽油加速大鼠肛周脓肿术后创面修复的作用机制研究

Mechanism of compound seabuckthorn seed oil accelerating postoperative wound repair of perianal abscess in rats

目的:探讨复方沙棘籽油加速大鼠肛周脓肿术后创面修复的作用机制。方法:取制备成功肛周脓肿术后创面SD模型鼠共50只,随机分为5组,分别为模型组(G1,给予蒸馏水);阳性对照组(G2,给予高锰酸钾);复方沙棘籽油纳米乳化液低剂量治疗组(G3);复方沙棘籽油纳米乳化液中剂量治疗组(G4);复方沙棘籽油纳米乳化液高剂量治疗组(G5);造模后各组实验大鼠创面用相应实验药物均匀涂抹,2次/d,连续给药14 d。观察治疗5 d、8 d、14 d后各组大鼠创面愈合情况,计算各组大鼠创面愈合率,分别取各组大鼠创面组织,应用免疫组化S-P法检测创面表皮生长因子(EGF)、磷酸化细胞外调节蛋白激酶(ERK)1/2、白细胞介素-8(IL-8)、前列腺素E2(PGE2)表达情况。结果:创面处理后5 d、8 d、14 d,G5组、G4组、G3组、G2组、G1组大鼠创面愈合率依次升高(均P<0.05)。术后14 d,大鼠创面IL-8表达水平为G2组<G5组<G4组<G3组<G1组(P<0.05);PEG2表达水平为G5组<G2组<G4组<G3组<G1组(P<0.05)。术后14 d,大鼠创面EGF、ERK1/2表达为G5组>G4组>G3组>G2组>G1组(均P<0.05)。结论:复方沙棘籽油纳米乳化液可有效促进大鼠肛周脓肿术后创面愈合,这可能与IL-8及PEG2炎症介质有关,还可能与EGF-ERK通路活化促进创面角质形成细胞、成纤维细胞生长、增殖、分化有关。

Objective To study the effect of compound seabuckthorn seed oil on accelerating wound healing after perianal abscess surgery in rats.Methods A total of 50 SD model mice were used to successfully prepare postoperative wounds of perianal abscess.They were randomly divided into five groups:G1 model group(given distilled water);G2 positive control group(given potassium permanganate);G3 treatment group(low dose group of compound seabuckthorn seed oil nano-emulsion);G4 treatment group(middle dose group of compound seabuckthorn seed oil nano-emulsion);G5(high dose group of compound seabuckthorn seed oil nano-emulsion).After modeling,the wounds of experimental rats in each group were evenly smeared with corresponding experimental drugs twice a day for 14 days.Observe the wound healing of rats in each group after 5 d,8 d and 14 d of treatment,calculate the wound healing rate of rats in each group and take the wound tissue of rats in each group respectively,and analyze the expression of EGF,phosphorylated ERK1/2,IL-8 and PEG2 in the wound with immunohistochemical S-P method.Results The wound healing rate of rats in G5 group,G4 group,G3 group,G2 group and G1 group increased in turn at 5 d,8 d and 14 d after wound treatment(allP<0.05).14 days after surgery,IL-8 expression in rat wounds G2<G5<G4<G3<G1(P<0.05);PEG2 expressed G5<G2<G4<G3<G1(P<0.05).14 days after surgery,The expression of EGF and ERK1/2 in rat wounds was G5>G4>G3>G2>G1(allP<0.05).Conclusion Compound seabuckthorn seed oil nano-emulsion can effectively promote wound healing after perianal abscess surgery in rats,which may be related to the inflammatory mediators of IL-8 and PEG2,and its activation of"EGF-ERK"pathway can promote the growth,proliferation and differentiation of wound keratinocytes and fibroblasts.